Infections, Sepsis
Conditions
Brief summary
"Klebsiella pneumoniae is considered intrinsically resistant to ampicillin because of the chromosomal blaSHV β-lactamase. However, amoxicillin-susceptible isolates have been sporadically reported. This retrospective observational study analyzes amoxicillin-susceptible K. pneumoniae isolates collected between 2016 and 2023 at a university hospital in France. Whole-genome sequencing, phylogenetic analysis, and virulence and resistance profiling were performed, and clinical data were reviewed. The study aims to characterize the genomic mechanisms underlying amoxicillin susceptibility and to assess associations with specific lineages, particularly hypervirulent clonal complex 66 (CC66), and clinical features."
Interventions
BIOLOGICALBacterial species identification by Whole Genome sequencing
Sponsors
Centre Hospitalier Universitaire de Nice
Study design
Observational model
CASE_ONLY
Time perspective
RETROSPECTIVE
Eligibility
Sex/Gender
ALL
Healthy volunteers
No
Inclusion criteria
* Patients with confirmed Klebsiella pneumoniae infection. * Isolates collected between 2016 and 2023. * Isolates with confirmed susceptibility to amoxicillin.
Exclusion criteria
* Isolates not confirmed as Klebsiella pneumoniae. * Isolates without antimicrobial susceptibility confirmation.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Assessment of blaSHV gene status (presence, deletion, or disruptive mutations) by whole-genome sequencing and its association with amoxicillin susceptibility. | At isolate collection and genomic analysis (2016-2023) | Whole-genome sequencing was used to determine blaSHV gene status (presence, absence, or disruptive mutations) |
Countries
France
Outcome results
None listed