Vascular Aging
Conditions
Keywords
SNRK, carotid atherosclerosis, vascular endothelial cells, cell senescence, vascular aging
Brief summary
Cardiovascular diseases pose a serious threat to public health, and their prevalence is on the rise year by year. Vascular aging is an independent risk factor for cardiovascular diseases, and endothelial cell senescence is an early event in vascular aging. Its occurrence can lead to endothelium-dependent vasodilation dysfunction, reduced vascular permeability, and the release of the senescence-associated secretory phenotype (SASP). These vascular pathological changes further damage the vascular media, leading to vascular remodeling and reduced compliance, accelerating the progression of atherosclerosis, and ultimately resulting in cardiovascular diseases such as coronary heart disease and hypertension. Recent research of the investigators has revealed that SNRK, a new member of the AMPK family of cellular energy sensors, plays a key regulatory role in vascular development. Based on this finding, the investigators propose the scientific hypothesis that SNRK responds to both physiological and pathological aging stimuli through differential mechanisms and regulates the process of endothelial cell senescence. In this study, the investigators will explore the correlation between SNRKAS and carotid vascular structure and endothelial function by measuring the levels of the SNRK upstream lncRNA (SNRKAS) in participants' peripheral blood, in conjunction with carotid ultrasound examinations. The findings will provide a solid scientific basis for elucidating new mechanisms underlying the onset and progression of vascular aging and for identifying novel therapeutic targets.
Interventions
DIAGNOSTIC_TESTserum tests and carotid ultrasound test
1. 10 mL of venous blood was drawn from each participant to measure blood lipids and serum levels of SNRKAS (using RT-qPCR), and to perform transcriptomic analysis. 2. Carotid ultrasound was used to measure circumferential strain and pulse wave velocity in both carotid arteries to assess the degree of arterial stiffness.
Sponsors
Tianjin Medical University
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
1. Aged 18-80 years, with the capacity to make decisions independently or represented by an authorized legal guardian; 2. Able to provide complete personal information, medical history, and lifestyle history (e.g., smoking and alcohol consumption history); 3. No history of severe cardiovascular disease, and deemed eligible for inclusion by a physician.
Exclusion criteria
1. Women who are pregnant or may become pregnant; 2. Patients with a history of neurological disorders, tumors, severe cardiovascular or pulmonary disease, liver failure, kidney failure, or blood disorders; 3. Patients who have undergone carotid stenting, carotid endarterectomy, or other similar procedures, or who have unilateral carotid artery occlusion due to any cause; 4. Patients who have participated in another clinical trial within the past 4 weeks; 5. Individuals deemed unsuitable for this clinical trial by the investigators.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Serum levels of SNRKAS detected by RT-PCR | At enrollment | serum levels of SNRKAS (fold change) |
| Degree of bilateral carotid artery stenosis detected by Doppler ultrasound | At enrollment | Intima-media thickness (mm) and lumen diameter (mm) of bilateral common carotid artery and internal carotid artery |
| Pulse wave velocity in both carotid arteries detected by Doppler ultrasound | At enrollment | Peak systolic velocity (cm/s) of bilateral common carotid artery and internal carotid artery |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Blood lipids levels detected by automated biochemical analyzer (LABOSPECT 008, Hitachi) | At enrollment | Total Cholesterol (mmol/L), Triglycerides (mmol/L), High-Density Lipoprotein (mmol/L), Low-Density Lipoprotein (mmol/L) |
Countries
China
Contacts
CONTACTXintong Ge, M.D.
STUDY_CHAIRMinghui Zou, Ph.D.
mhzou@tmu.edu.cn
Outcome results
None listed