Overweight, Type 1 Diabetes
Conditions
Keywords
Type 1 diabetes, Exercise training, healthy controls, overweight
Brief summary
The purpose of this study is: * To see if insulin resistance (how sensitive your muscle tissue is to insulin) is associated with lower cardio fitness in people with Type 1 diabetes compared to healthy controls, before and after a High Intensity Interval Training (HIIT) exercise program. * To see if being overweight and having Type 1 diabetes is associated with lower cardio fitness compared to overweight healthy controls, before and after a HIIT exercise program.
Detailed description
This study will test the hypothesis that in humans with T1D skeletal muscle microvascular insulin resistance predicts a reduction in Cardiorespiratory Fitness (CRF) and obesity exacerbates microvascular insulin resistance, leading to further CRF decline, which is mechanistically linked to increased muscle microvascular endothelial oxidative stress, impaired muscle mitochondrial function, reduced muscle capillary density and decreased satellite cells.
Interventions
OTHERExercise training
15 weeks of HIIT exercise training
Sponsors
University of Virginia
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
21 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* • Male or female ≥21 and ≤50 years old * For persons with T1D: Disease duration ≥ 5 years and HbA1c ≤ 8.5% on multiple daily insulin injection or insulin pump * Body mass index: ≥19 and ≤27 kg/m2 for control and T1D, ≥30 and ≤40 kg/m2 (27.5 to 37.5 for Asian Americans) for obesity and T1D + obesity. BMI is limited to ≤40 kg/m2 (37.5 for Asian Americans) for easier vascular access and cardiac imaging. * Stable use of non-insulin medications for over 6 months other than estrogen/progesterone containing medications which must be discontinued at least 3 months prior to the study (intrauterine devices may be continued due to limited systemic absorption)
Exclusion criteria
* • Acute or chronic disease other than T1D or obesity * History of microvascular or macrovascular diabetes complications * History of diabetic ketoacidosis in the past 24 months * History of hypoglycemia unawareness * Recently active (\>20 min of moderate/high intensity exercise, 2 times/week) * Subjects who are smokers or who have quit smoking \<5 years * Subjects with hypertriglyceridemia (\>400 mg/dl) * Current use of vasoactive medications (i.e. calcium channel blockers, angiotensin-converting enzyme or renin inhibitors, angiotensin-receptor blockers, nitrates, alpha- or beta-blockers, or diuretics). * Females taking oral contraceptives in the past 3 months * Subjects with a history of significant metabolic, cardiac, cerebrovascular, hematological, pulmonary, gastrointestinal, liver, renal, or endocrine disease or malignancy * Pregnant (as evidenced by positive pregnancy test) or nursing women * Musculoskeletal condition preventing participation in exercise testing or exercise training * History of gastroparesis * Pulse oximetry \<90%
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Microvascular blood volume in skeletal and cardiac muscle - changes from baseline | Before and after 15 weeks of exercise training | insulin-mediated changes in skeletal and cardiac muscle as determined by insulin clamp |
| maximal aerobic exercise capacity | before and after 15 weeks of exercise training | change in VO2max |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| insulin-mediated whole body glucose disposal | before and after 15 weeks of exercise training | insulin-mediated whole body glucose disposal during insulin clamp |
| Muscle capillary density and satellite cells | before and after 15 weeks of exercise training | Changes in muscle capillary density and satellite cells |
Countries
United States
Contacts
CONTACTLee Hartline, MEd
CONTACTZhenqi Liu, MD
PRINCIPAL_INVESTIGATORZhenqi Liu, MD
University of Virginia
Outcome results
None listed