Exercise, Weight Loss
Conditions
Keywords
Complete Blood Count, Exercise, Flow Cytometry, Lymphocyte Subsets, Immune System, Rapid Weight Loss
Brief summary
Wrestling is a weight-classified sport in which athletes frequently apply rapid weight loss (RWL) strategies prior to competition. While the performance-related consequences of RWL have been widely investigated, its combined effects with exercise on hematological parameters and immune cell profiles remain insufficiently understood. Therefore, this study aims to examine the effects of short-term weight loss and exercise on whole blood cell parameters and lymphocyte subsets in elite wrestlers. Nine elite male wrestlers actively competing in the senior category (mean age: 22.90 ± 5.15 years; mean height: 1.75 m) voluntarily participated in this study. Athletes will achieve an average body mass reduction of 4.3% over a five-day period. Venous blood samples will be collected at 09:30 a.m. in a fasted state during two experimental phases: (1) Exercise-only phase, with blood sampling before and after exercise (BRWL1 and BRWL2); (2) Exercise plus weight loss phase, following the five-day weight loss period, with blood sampling before and after exercise (ARWL1 and ARWL2). Complete blood count parameters will be analyzed using an automated hematology analyzer, while lymphocyte subsets (CD3+, CD4+, CD8+, CD19+, CD16/56+) will be assessed by flow cytometry. Statistical analyses will be performed with a significance level set at p \< 0.05.
Detailed description
See brief summary for study protocol details
Interventions
OTHERExercise
Standardized wrestling exercise session; pre- and post-exercise venous blood collected
OTHERRapid Weight Loss
5-day rapid weight loss protocol achieving average 4.3% body mass reduction
Sponsors
Ankara Yildirim Beyazıt University
Study design
Allocation
NON_RANDOMIZED
Intervention model
SEQUENTIAL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* Being between 18 and 35 years of age * Being clinically healthy * Actively competing in wrestling * Having regular training experience * No history of chronic disease * No musculoskeletal injury that could affect performance * Not using any medication or nutritional supplements that could influence hematological or biochemical parameters during the study period * Willing to volunteer and provide written informed consent
Exclusion criteria
* Having any chronic disease (e.g., cardiovascular, metabolic, respiratory, or neurological disorders) * Having a recent musculoskeletal injury that could affect exercise performance * Using medications, ergogenic aids, or nutritional supplements that might influence hematological or biochemical parameters * Having a history of smoking or alcohol abuse * Failing to complete the testing procedures and training protocol required for the study * Not providing written informed consent * Withdrawing voluntarily during the study period
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in Leukocyte Count | Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group) | Change in total white blood cell (WBC) count measured from pre- to post-exercise |
| Change in Lymphocyte Percentage | Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group) | Change in percentage of lymphocytes relative to total WBC count, measured from pre- to post-exercise. |
| Change in CD3+ T Cell Count | Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group) | Change in absolute count of CD3+ T lymphocytes measured from pre- to post-exercise. |
| Change in CD4+ T Cell Count | Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group) | Change in absolute count of CD4+ helper T lymphocytes measured from pre- to post-exercise. |
| Change in CD8+ T Cell Count | Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group) | Change in absolute count of CD8+ cytotoxic T lymphocytes measured from pre- to post-exercise. |
| Change in CD19+ B Cell Count | Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group) | Change in absolute count of CD19+ B lymphocytes measured from pre- to post-exercise. |
| Change in CD16/56+ NK Cell Count | Pre- and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group) | Change in absolute count of CD16/56+ natural killer (NK) cells measured from pre- to post-exercise. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Change in Creatine Kinase and Lactic Acid Levels | Pre-exercise and post-exercise (Day 1 for Exercise Only group; Day 5 for Exercise + Rapid Weight Loss group) | Serum creatine kinase (CK) and lactic acid (LA) levels measured to assess anaerobic performance and muscle damage. |
Countries
Turkey (Türkiye)
Outcome results
None listed