Protein Metabolism
Conditions
Brief summary
The purpose of this study is to assess the digestion and absorption kinetics of beef consumed alone or as part in healthy adults. In a randomized, crossover design, 10 young, healthy adult males and females aged 18-35 years, will consume a meal consisting of 25 g protein from minced beef alone or as part of a mixed-meal during two separate experimental trials. The main study endpoint is the exogenous rate of amino acid appearance in response to the ingested beef over a 10-h period.
Interventions
DIETARY_SUPPLEMENTFood proteins
Meal consisting of 25 g protein･kg BM-1 from minced beef protein.
Sponsors
Maastricht University Medical Center
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
Within-subject, randomized, crossover design
Eligibility
Sex/Gender
ALL
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* Aged between 18-35 years * BMI between 18.5-30 kg/m2 * Healthy, recreationally active (exercise at least once per two weeks and a maximum of four days per week) * No physical limitations (i.e., able to perform all activities associated with daily living independently)
Exclusion criteria
* Smoking * Vegan, vegetarian, or does not consume beef protein * Diagnosed musculoskeletal disorders * Use of any medications known to affect protein metabolism (i.e., corticosteroids, non-steroidal anti-inflammatories, or prescribed acne medications) * Chronic use of gastric acid-suppressing medication or anti-coagulants * Unstable weight over the last three months * Diagnosed GI tract disorders or diseases * Blood donation in the past 2 months * Females: pregnancy
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Digestion and absorption kinetics | Over the 10 hour timeframe following beef ingestion | The exogenous rate of amino acid appearance in response to the ingested beef (expressed as μmol/kg/min). Based on tracer plasma amino acid concentrations over time (measured with ultra performance liquid chromatography-mass spectrometry). |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Whole-body protein metabolism | Over the 10 hour timeframe following beef ingestion | Whole-body protein metabolism includes measurements of protein synthesis, breakdown, oxidation, and net balance (all expressed as μmol/kg/h). Based on amino acid concentrations in plasma and breath samples over time (measured with ultra performance liquid chromatography-mass spectrometry and gas chromatography-isotope ratio mass spectrometry). |
Countries
Netherlands
Contacts
CONTACTJulia M Malowany, MSc
CONTACTJorn Trommelen, PhD
PRINCIPAL_INVESTIGATORLuc JC van Loon, PhD
Maastricht University
Outcome results
None listed