Protein Metabolism
Conditions
Brief summary
The purpose of this study is to assess the amino acid oxidation dose-response relationship of ingested whole-food protein in healthy adults. In a randomized, crossover design, 20 young, healthy adult males and females aged 18-35 years, will consume a dinner consisting of 0.32, 0.74, and 1.32 g protein･kg BM-1 (corresponding to 1, 2, and 4 minced beef patties, respectively) during 3 separate experimental trials.The main study endpoint is the amino acid oxidation of ingested protein over a 24-h period.
Interventions
DIETARY_SUPPLEMENTFood proteins
Dinner meal consisting of minced beef protein.
Sponsors
Maastricht University Medical Center
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
Within-subject, randomized, crossover design
Eligibility
Sex/Gender
ALL
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* Aged between 18-35 years * BMI between 18.5-30 kg/m2 * Healthy, recreationally active (exercise at least once per two weeks and a maximum of four days per week) * No physical limitations (i.e., able to perform all activities associated with daily living independently)
Exclusion criteria
* Smoking * Vegan, vegetarian, or does not consume beef protein * Diagnosed musculoskeletal disorders * Use of any medications known to affect protein metabolism (i.e., corticosteroids, non-steroidal anti-inflammatories, or prescribed acne medications) * Chronic use of gastric acid-suppressing medication or anti-coagulants * Unstable weight over the last three months * Diagnosed GI tract disorders or diseases * Blood donation in the past 2 months * Females: pregnancy
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Amino acid oxidation of ingested protein | Over the 24 hour timeframe following protein ingestion | Amino acid oxidation; compared between interventions to determine the dose-response relationship of amino acid oxidation to ingested whole-food protein. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Plasma amino acid response of ingested protein | Over the 24 hour timeframe following protein ingestion | Plasma amino acid response; comparison between interventions to determine the plasma amino acid response to different amounts of ingested whole-food protein. |
Countries
Netherlands
Contacts
CONTACTJulia M Malowany, MSc
CONTACTJorn Trommelen, PhD
PRINCIPAL_INVESTIGATORLuc JC van Loon, PhD
Maastricht University
Outcome results
None listed