Periodontitis
Conditions
Brief summary
This study aimed to investigate gingival crevicular fluid (GCF) and serum soluble MerTK (sMerTK) and Gas6 levels in periodontal health and disease. A total of 90 individuals, 30 patients with stage III grade B periodontitis, 30 with gingivitis and 30 periodontally healthy individuals were included. Whole-mouth and site-specific clinical periodontal parameters including probing depth, clinical attachment level, bleeding on probing, gingival index, plaque index were recorded. GCF and serum sMerTK and Gas6 levels were measured by enzyme-linked immunosorbent assay. Statistical analysis was performed by using non-parametric tests.
Interventions
Gingival crevicular fluid collection using sterile paper strips inserted into the gingival sulcus for 30 seconds from selected periodontal sites (healthy, gingivitis, and periodontitis) for biomarker analysis (Gas6 and sMerTK).
Sponsors
Abant Izzet Baysal University
Study design
Observational model
OTHER
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Individuals without systemic diseases that may affect periodontal tissues (such as cardiovascular diseases, atherosclerosis, immune system disorders, rheumatoid arthritis, obesity, cancer, and diabetes) * Individuals who have not used antibiotics within the last 3 months * Individuals not taking any medications known to affect periodontal tissues * Individuals without infectious diseases such as HIV, hepatitis B virus (HBV), or tuberculosis * Individuals with at least 16 natural teeth, excluding third molars * Individuals aged 18 years and older
Exclusion criteria
* Pregnant or lactating individuals * Individuals who have received periodontal treatment within the last 6 months * Current smokers
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Gas6 Levels in Gingival Crevicular Fluid | baseline | Quantitative measurement of Gas6 levels (pg/mL) in gingival crevicular fluid samples using enzyme-linked immunosorbent assay (ELISA), and comparison of these levels among periodontally healthy, gingivitis, and periodontitis groups. |
| Soluble MerTK (sMerTK) Levels in Gingival Crevicular Fluid | baseline | Quantitative measurement of soluble MerTK (sMerTK) levels (pg/mL) in gingival crevicular fluid samples using enzyme-linked immunosorbent assay (ELISA), and comparison of these levels among periodontally healthy, gingivitis, and periodontitis groups. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Plaque Index | baseline | Measurement of plaque index (score) in participants with periodontal health, gingivitis, and periodontitis, and evaluation of its correlation with gingival crevicular fluid Gas6 and soluble MerTK levels. |
| Probing Depth | baseline | Measurement of probing depth (mm) in participants with periodontal health, gingivitis, and periodontitis, and evaluation of its correlation with gingival crevicular fluid Gas6 and soluble MerTK levels. |
| Clinical Attachment Loss | baseline | Measurement of clinical attachment loss (mm) in participants with periodontal health, gingivitis, and periodontitis, and evaluation of its correlation with gingival crevicular fluid Gas6 and soluble MerTK levels. |
| Gingival Index | baseline | Measurement of gingival index (score) in participants with periodontal health, gingivitis, and periodontitis, and evaluation of its correlation with gingival crevicular fluid Gas6 and soluble MerTK levels. |
Countries
Turkey (Türkiye)
Outcome results
None listed