Healthly Volunteers
Conditions
Keywords
rutin, Ergogenic aids, Oxidative stress, 40km time trial
Brief summary
Background: Rutin has several health benefits like improving total antioxidant capacity(TAC), inducing more nitric oxide(NO) produced from endothelium, and anti-inflammatory. Studies show rutin can enhance sport performance in mouse, but there isn't any human trail suggests that rutin can improve sport performance in high-stressed state. Purpose: This study want to investigated the effects of rutin on oxidative stress, inflammatory markers, and blood flow velocity in 40km time trial. Method: A single-blinded crossover placebo-controlled study design is used and rutin supplementation is compared to a non-treatment control group. Twelve subjects are randomly assigned into two groups supplemented with the dose of 2000mg/day rutin or placebo for two weeks. All subjects participate 40km cycling time trails(TT) after rutin or placebo supplement. Venous blood samples are drawn before meal, before exercise and immediately after the exercise in order to estimate malondialdehyde(MDA) levels, TAC, Interleukin 10(IL-10), tumor necrosis factor-alpha(TNF-a), and high-sensitivity C-reactive protein(HS-CRP). In TT, blood flow velocity is monitored by near infrared spectroscopy(NIRS) and HR rating of perceived exertion(RPE) and rating-of-fatigue (ROF) scale are also recorded. Expected results:14 days rutin supplementation can improve 40km TT performance by reducing exercise-induced oxidative stress and enhancing blood flow velocity in healthy young adults.
Interventions
The placebo capsule supplement was taken for 14 days (4 capsules per day)
OTHERRutin (Rutin trial)
The rutin capsule supplement was taken for 14 days, with a daily dosage of 2000 mg of rutin (equivalent 4 to capsules per day, each containing 500 mg of rutin).
Sponsors
China Medical University Hospital
National Science and Technology Council
China Medical University, China
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
OTHER
Masking
QUADRUPLE (Subject, Caregiver, Investigator, Outcomes Assessor)
Intervention model description
Twelve young healthy physically active male college students. Participants were crossed over for rutin or placebo supplements, which was completed in fourteen days. Between the crossover trials, participants had a two-week washout period to nullify the effects of the supplement.
Eligibility
Sex/Gender
MALE
Age
18 Years to 25 Years
Healthy volunteers
Yes
Inclusion criteria
* Recruit people who are healthy are 18-25 years male and have regular exercise habits in college students
Exclusion criteria
* Have smoking and drinking habits. * Those who have implanted artificial joints in the past six months and have had recent surgery. * People who feel unwell due to other reasons during the experiment. * Take any drugs or Nutrition supplements in the past 3 months.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| 40 km cycling time trial | 14 days | Investigators conducted a double-blind test, where volunteers' basal maximal oxygen consumption (VO2max) was measured both before the intervention. The pretest VO2max is a reference to adjust the exercise intensity for each individual. To assess volunteers' aerobic endurance capacity by 40km cycling time trail(40km TT), participants exercised at 55% VO2max, and the cycling time from the start to 40km was recorded. |
| Near infrared spectroscopy | 14 days | Near infrared spectroscopy(NIRS)(Moxy, Fortiori Design LLC, Minnesota, USA) can non-invasively observe blood oxygenation and blood flow through infrared light. Before the exercise, NIRS was fixed on the subject's thigh. During 40km time trial, blood flow velocity was collected by NIRS. |
| Rating-of-fatigue (ROF) scale | 14 days | The Rating of Fatigue Scale measures perceived fatigue during or after exercise. Participants rate fatigue from 0 (no fatigue) to 10 (extreme fatigue). A higher score indicates greater fatigue (worse condition). |
| The Borg Rating of Perceived Exertion(RPE) Scale | 14 days | The Borg Rating of Perceived Exertion Scale measures how hard exercise feels. Participants rate effort from 6 (no exertion) to 20 (maximal exertion). A higher score means greater effort (worse fatigue). |
| Serum Tumor Necrosis Factor-alpha (TNF-alpha) Concentration | 14 days | A pro-inflammatory cytokine measured in serum using a commercial ELISA kit (BioLegend) to evaluate the inflammatory response.Unit of Measure: Picograms per milliliter (pg/mL) |
| Superoxide Dismutase (SOD) Activity | 14 days | biomarker used to evaluate total antioxidant capacity. Serum SOD activity is measured using a commercial colorimetric assay kit and read with an ELISA plate reader.Unit of Measure: Units per milliliter (U/mL). |
| Lactate Dehydrogenase (LDH) Activity | 14 days | A biomarker of muscle damage measured in serum using an automated clinical chemistry analyzer (Beckman Coulter AU5800). Unit of Measure: Units per Liter (U/L) |
| Serum Creatine Kinase (CK) Concentration | 14 days | A biomarker of muscle damage measured in serum using an automated clinical chemistry analyzer (Beckman Coulter AU5800). Unit of Measure: Units per Liter (U/L) |
| Malondialdehyde (MDA) Levels | 14 days | A standard biomarker of lipid peroxidation. Serum MDA levels are determined using a commercial ELISA kit to assess oxidative stress status. Unit of Measure: micromolar (μM) |
| High-sensitivity C-reactive Protein(HS-CRP) Concentration | 14 days | A biomarker of inflammation measured in serum using enzyme-linked immunosorbent assay (ELISA) with a microplate reader. Unit of Measure: milligrams per liter (mg/L). |
| Uric Acid (UA) Levels | 14 days | Measured in serum as a marker of metabolic stress and muscle damage using commercial analytical reagents. Unit of Measure: Milligrams per deciliter (mg/dL). |
| Serum Interleukin-10 (IL-10) Concentration | 14 days | Measured in serum as a marker of metabolic stress and muscle damage using commercial analytical reagents. Unit of Measure: Milligrams per deciliter (mg/dL). |
Countries
Taiwan
Contacts
STUDY_CHAIRJung-Piao Tsao
China Medical University, China
Outcome results
None listed