Rheumatoid Arthritis, Systemic Lupus Erythematosus, Systemic Sclerosis, Healthy Adult Volunteers
Conditions
Keywords
Autoimmune disease, multi-omic profiling, immunology
Brief summary
This prospective cohort study aims to constitute a 500-participant database and biobank including 450 adults with systemic autoimmune diseases (rheumatoid arthritis, systemic lupus erythematosus, systemic sclerosis) and 50 healthy controls.
Detailed description
The Immun4Cure Cohort is a monocentric prospective cohort conducted at Montpellier University Hospital. Autoimmune diseases (AIDs) are complex, heterogeneous conditions involving dysregulation of innate and adaptive immunity, chronic inflammation, and irreversible tissue damage. The three targeted diseases-rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), and systemic sclerosis (SSc represent major public-health challenges due to their severity, treatment burden, and impact on quality of life. The study integrates standardized longitudinal clinical follow-up over 5 years, deep phenotyping, multi-omic analyses (genomics, transcriptomics, proteomics, metabolomics, immunophenotyping), environmental exposure assessment, and biological sample collection (blood, serum, plasma, PBMCs, urine, stool, saliva, optional tissue biopsies). Healthy subjects undergo baseline and 12-month evaluations to establish reference immune, metabolic, and environmental signatures. This cohort will serve as a foundational research platform for translational studies and ancillary projects on autoimmune diseases.
Interventions
BIOLOGICALOptional collection
The optional collection will include: * stool sample (50 mL) * urine sample (50 mL) * saliva sample (2 mL)
BIOLOGICALBlood test and principal collection
A total of 62.5 mL of blood will be collected while fasting, using the following tubes: * one 2.5 mL PAXGene Blood RNA Tube * seven 6mL heparinised Tubes * two 6mL EDTA Tubes * one 6mL dry Tube As part of routine care for groups 1, 2, and 3, and specific to research for group 4: A biological research assessment (urine and blood): complete blood count, platelets, HbA1c, reticulocytes, schizocytes, haptoglobin, ionogram, troponin, NT-proBNP, calcium, CRP, creatinine, GFR, uric acid, ferritin, CPK, fasting blood glucose, albumin, serum protein electrophoresis, total bilirubin, γGT, ASAT, ALAT, PAL, TSH, total cholesterol, LDL, HDL, triglycerides, B9, B12, HBV, HCV, HIV, CMV, EBV serology, antinuclear antibodies, DNA, ACPA, rheumatoid factor, C3, C4, proteinuria/creatininuria, urine strip test
For groups 1 to 3 : Self-questionnaires will assess factors such as medical history, comorbidity exposure to toxic substances, occupational exposures, psychological and clinical factors. occupation and household income, as well as lifestyle habits (tobacco and alcohol consumption, possible drug use, sexual activity). Anxiety and depression environmental factors throughout life (diet, physical activity, sleep, contraception, sun exposure, pollution, noise, proximity to green spaces, etc.), 3-day food diary For group 4: Self-questionnaires will assess factors such comorbidity exposure to toxic substances, occupational exposures, psychological and clinical factors. occupation and household income, as well as lifestyle habits (tobacco and alcohol consumption, possible drug use, sexual activity). Anxiety and depression environmental factors throughout life (diet, physical activity, sleep, contraception, sun exposure, pollution, noise, proximity to green spaces, etc.), 3-day food diary
OTHERExamination
Group 1-RA: Capillaroscopy and optionnal Synovial microbiopsy Group 2-SLE: Optical coherence tomography (OCT) and optionnal skin biopsy and optionnal capillaroscopy Group 3-SSc: Capillaroscopy and optionnal skin biopsy Group 4: Optical coherence tomography (OCT) and capillaroscopie. Optionnal skin biopsy and optionnal Synovial microbiopsy
Sponsors
University Hospital, Montpellier
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
Participants: Group 1: RA * Adults ≥18 years * Patient followed as part of their MAI in one of the departments participating in the study at UHM * Confirmed diagnosis according to international criteria :ACR/EULAR 2010 Group 2: LES * Adults ≥18 years * Patient followed as part of their MAI in one of the departments participating in the study at UHM * Confirmed diagnosis according to international criteria :ACR/EULAR 2019 Group 3: SSc * Adults ≥18 years * Patient followed as part of their MAI in one of the departments participating in the study at UHM * Confirmed diagnosis according to international criteria :ACR/EULAR 2013 Group 4: Healthy Controls * Adults ≥18 years * No symptoms of autoimmune disease * No first-degree family history of autoimmune disease
Exclusion criteria
(all groupes): * Patients who have refused or are unable to give informed consent * Inability to follow the subject during the study period * Participation in another interventional study that includes an exclusion period that is still ongoing * Pregnant women * Not affiliated with a social security scheme * Patients without a national insurance number * Persons under judicial protection, guardianship or trusteeship * Persons deprived of their liberty
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| AUC-ROC discriminant performance of multi-omic biomarkers | Baseline to 60 months | Discriminatory power (AUC-ROC) of biological markers measured using a multi-omic approach (genomics, proteomics, transcriptomics, epitranscriptomics, immunology, metabolomics) common to adult patients with autoimmune disease (AID), compared to adults without AID. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Characterization of Pathophysiological Mechanisms Through Multi-Omic and Spatial Immune Profiling | Baseline, disease flare (if applicable), and end of follow-up (Month 60) | Immune signatures from blood and tissue samples (including synovial, skin, and other available biopsies) will be analyzed to determine disease-specific patterns and their discriminative capacity across autoimmune diseases. Immune cells, particularly macrophages, will be isolated and profiled using single-cell-based spatial analyses. Samples will undergo multi-omic characterization, including proteomic, transcriptomic, epitranscriptomic, immunological, and metabolomic analyses, to identify disease-specific molecular and cellular signatures. |
Contacts
CONTACTCharlotte KAAN
STUDY_DIRECTORYves-Marie PERS, Pr
Montpellier University Hospital
STUDY_DIRECTOREdouard TUAILLON, Pr
Montpellier University Hospital
Outcome results
None listed