Colistin Resistance in Bacteria, Patients With Multi Drug Resistant Gram-negative Bacteria
Conditions
Keywords
colistin resistant, multi drug resistant gram-negative bacteria, broth microdilution, disk elution test
Brief summary
* Evaluation of the disk elution test as reliable routine method for detection of colistin resistant among multidrug resistant gram-negative bacteria compared with broth microdilution. * Molecular detection of colistin resistant in multidrug-resistant gram-negative bacteria.
Interventions
DIAGNOSTIC_TESTdisk elution test
* Identification of microorganism using conventional methods or Vitek 2 compact system. * Anti biotic susceptibility test using conventional methods or Vitek 2 compact system. * For MDR gram negative bacteria: disc elution test and colistin broth microdilution
Sponsors
Assiut University
Study design
Observational model
OTHER
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Healthy volunteers
No
Inclusion criteria
-Multidrug-resistant gram-negative isolates will be collected from clinical specimens (blood, urine, sputum, wound swabs, etc.) obtained from hospitalized patients in Assiut University Hospitals.
Exclusion criteria
* gram-positive bacteria * carbapenem-sensitive gram-negative bacteria * repeated sample for same patient or different samples from the same patient
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Evaluation of the disk elution test for detection of colistin resistant among multidrug resistant gram-negative bacteria compared with broth microdilution. And Molecular detection of colistin resistant in multidrug-resistant gram-negative bacteria by PCR | start from 2026 up to 2029 |
Contacts
CONTACTchristen william, Master's degree in clinic path
Outcome results
None listed