Non-infectious Uveitis, Periodontal Disease
Conditions
Keywords
Cytokines, Gingival crevicular fluid, serum, saliva, Periodontal status, non-infectious uveitis
Brief summary
This cross-sectional observational study aimed to evaluate periodontal clinical parameters and inflammatory cytokine levels in gingival crevicular fluid (GCF), saliva, and serum in patients with non-infectious uveitis (NIU) compared with systemically healthy individuals. Twenty-five patients diagnosed with non-infectious uveitis and twenty-seven age- and sex-matched healthy controls were included. Clinical periodontal parameters including probing pocket depth, clinical attachment loss, plaque index, gingival index, and bleeding on probing were recorded. Biological samples including saliva, gingival crevicular fluid, and serum were collected. Levels of TNF-α, IL-1β, IL-2, IL-6, IL-8, IL-10, and IL-17 were measured using ELISA. The study aimed to investigate whether periodontal inflammatory status and systemic cytokine profiles are associated with non-infectious uveitis.
Detailed description
Non-infectious uveitis (NIU) is an immune-mediated inflammatory condition affecting the uveal tract of the eye and may be associated with systemic immune dysregulation. Periodontal disease is a chronic inflammatory disease characterized by destruction of the supporting tissues of the teeth and is known to contribute to systemic inflammatory burden through the release of cytokines and other inflammatory mediators. Both NIU and periodontal disease involve complex immune responses and cytokine networks. However, the potential relationship between periodontal inflammation and NIU remains unclear. The present cross-sectional study was designed to investigate possible associations between periodontal clinical status and systemic and local inflammatory cytokine profiles in patients with NIU. Participants included patients diagnosed with non-infectious uveitis and systemically healthy control individuals. Periodontal examinations were performed and biological samples including gingival crevicular fluid, saliva, and serum were collected. Levels of TNF-α, IL-1β, IL-2, IL-6, IL-8, IL-10, and IL-17 were measured using enzyme-linked immunosorbent assay (ELISA). The objective of this study was to compare periodontal parameters and cytokine levels between NIU patients and healthy controls in order to evaluate whether periodontal inflammatory burden is associated with systemic immune alterations in individuals with non-infectious uveitis.
Interventions
None listed
Sponsors
Selcuk University
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Participants aged 18 years or older. * Patients diagnosed with non-infectious uveitis according to the * Standardization of Uveitis Nomenclature (SUN) criteria. * Presence of at least 20 natural teeth. * Individuals who agreed to participate in the study and provided informed consent.
Exclusion criteria
* Presence of infectious uveitis. * Pregnancy or lactation. * Use of antibiotics, anti-inflammatory drugs, or immunosuppressive therapy within the previous 6 months. * History of periodontal treatment within the previous 6 months. * Presence of systemic diseases known to affect periodontal status. * Current smokers.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| IL-10 concentration in serum (pg/L) | Baseline | IL-10 concentration was measured in serum samples using enzyme-linked immunosorbent assay (ELISA). Cytokine concentrations were calculated based on standard curves and expressed as picograms per liter (pg/L). |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| IL-6 concentration in gingival crevicular fluid (ng/sec) | Baseline | IL-6 concentration was measured in gingival crevicular fluid samples using ELISA and expressed as nanograms per second (ng/sec). |
| IL-6 concentration in saliva (ng/min) | Baseline | IL-6 concentration was measured in saliva samples using ELISA and expressed as nanograms per minute (ng/min). |
| TNF-α concentration in gingival crevicular fluid (ng/sec) | Baseline | TNF-α concentration was measured in gingival crevicular fluid samples using ELISA. Total cytokine amount was calculated by multiplying concentration by sample volume and expressed as nanograms per second (ng/sec). |
| TNF-α concentration in saliva (ng/min) | Baseline | TNF-α concentration was measured in saliva samples using ELISA. Salivary cytokine amounts were adjusted for salivary flow rate and expressed as nanograms per minute (ng/min). |
| TNF-α concentration in serum (ng/L) | Baseline | TNF-α concentration was measured in serum samples using ELISA and expressed as nanograms per liter (ng/L). |
| IL-1β concentration in gingival crevicular fluid (pg/sec) | Baseline | IL-1β concentration was measured in gingival crevicular fluid samples using ELISA. Total cytokine amount was calculated by multiplying concentration by sample volume and expressed as picograms per second (pg/sec). |
| IL-1β concentration in saliva (pg/min) | Baseline | IL-1β concentration was measured in saliva samples using ELISA. Salivary cytokine amounts were adjusted for salivary flow rate and expressed as picograms per minute (pg/min). |
| IL-1β concentration in serum (pg/L) | Baseline | IL-1β concentration was measured in serum samples using ELISA and expressed as picograms per liter (pg/L). |
| IL-2 concentration in gingival crevicular fluid (ng/sec) | Baseline | IL-2 concentration was measured in gingival crevicular fluid samples using ELISA and expressed as nanograms per second (ng/sec). |
| IL-2 concentration in saliva (ng/min) | Baseline | IL-2 concentration was measured in saliva samples using ELISA and expressed as nanograms per minute (ng/min). |
| IL-6 concentration in serum (ng/L) | Baseline | IL-6 concentration was measured in serum samples using ELISA and expressed as nanograms per liter (ng/L). |
| IL-8 concentration in gingival crevicular fluid (ng/sec) | Baseline | IL-8 concentration was measured in gingival crevicular fluid samples using ELISA and expressed as nanograms per second (ng/sec). |
| IL-8 concentration in saliva (ng/min) | Baseline | IL-8 concentration was measured in saliva samples using ELISA and expressed as nanograms per minute (ng/min). |
| IL-8 concentration in serum (ng/L) | Baseline | IL-8 concentration was measured in serum samples using ELISA and expressed as nanograms per liter (ng/L). |
| IL-10 concentration in gingival crevicular fluid (pg/sec) | Baseline | IL-10 concentration was measured in gingival crevicular fluid samples using ELISA and expressed as picograms per second (pg/sec). |
| IL-10 concentration in saliva (pg/min) | Baseline | IL-10 concentration was measured in saliva samples using ELISA and expressed as picograms per minute (pg/min). |
| IL-17 concentration in gingival crevicular fluid (ng/sec) | Baseline | IL-17 concentration was measured in gingival crevicular fluid samples using ELISA and expressed as nanograms per second (ng/sec). |
| IL-17 concentration in saliva (ng/min) | Baseline | IL-17 concentration was measured in saliva samples using ELISA and expressed as nanograms per minute (ng/min). |
| IL-2 concentration in serum (ng/L) | Baseline | IL-2 concentration was measured in serum samples using ELISA and expressed as nanograms per liter (ng/L). |
| IL-17 concentration in serum (ng/L) | Baseline | IL-17 concentration was measured in serum samples using ELISA and expressed as nanograms per liter (ng/L). |
Countries
Turkey (Türkiye)
Contacts
PRINCIPAL_INVESTIGATORDuygu durmaz, DDS,MSc
Pendik Oral and Dental Health Hospital, Istanbul, Turkey
Outcome results
None listed