Biofilms, Dentures, Denture Stomatitis, Halitosis
Conditions
Brief summary
The goal of this observational study is to learn about denture wearers' knowledge, awareness, and self-reported practices regarding denture hygiene and oral probiotics, and to assess whether these are associated with clinical oral findings and microbial colonization in adult denture wearers (18 years and older), including complete and partial denture users treated at King Saud University Dental Clinics within the past five years. The main questions it aims to answer are: What are the levels of knowledge, awareness, and practices related to denture hygiene and oral probiotics among adult denture wearers? What is the prevalence of denture-related oral conditions and bacterial/fungal colonization, and how are these related to denture hygiene knowledge and self-reported hygiene practices? Participants will: Complete an electronic questionnaire in Arabic or English about demographics, denture hygiene knowledge, awareness of oral probiotics, and current denture care practices. Attend a 15-20 minute clinical visit for examination of dentures and oral mucosa. Be asked to avoid cleaning their denture for at least 12 hours before the visit. Undergo plaque sample collection from dentures and oral tissues; partially edentulous participants will also have additional samples collected from abutment areas. Have samples analyzed using real-time PCR and culture methods (CFU) to detect bacterial and fungal microorganisms.
Interventions
OTHERThis is a cross-sectional observational study. Participants are not assigned to any treatment or preventive intervention.
This is a cross-sectional observational study. Participants are not assigned to any treatment or preventive intervention. They will only undergo questionnaire completion, clinical examination, and noninvasive plaque and mucosal sample collection.
Sponsors
King Saud University
Study design
Observational model
CASE_ONLY
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
Adults 18 years or older Currently wearing at least one removable denture (complete or partial) Denture received/used within the past five years Willing and able to provide informed consent
Exclusion criteria
Severe systemic conditions affecting oral health, such as: Uncontrolled diabetes (HbA1c \> 7) and Immunosuppression Pregnancy Breastfeeding Cognitive or physical impairment that limits the ability to perform routine oral and denture care practices
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Knowledge, awareness, and self-reported hygiene practices related to denture hygiene and oral probiotics among adult denture wearers, assessed using a validated questionnaire | Baseline | Knowledge, awareness, and self-reported hygiene practices related to denture hygiene and oral probiotics among adult denture wearers, assessed using a validated questionnaire |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Prevalence of denture-related oral complications and bacterial/fungal colonization, assessed through clinical examination, culture (CFU), and real-time PCR | Baseline | Prevalence of denture-related oral complications and bacterial/fungal colonization, assessed through clinical examination, culture (CFU), and real-time PCR |
Countries
Saudi Arabia
Outcome results
None listed