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Mandibular Stretching and 4-4-8 Breathing Exercises on Autonomic Nervous System Activity

Investigation of the Effects of Mandibular Stretching Exercise and 4-4-8 Breathing Exercise on Autonomic Nervous System Activity, Muscle Tone, and Psychological State: A Randomized Controlled Trial

Status
Recruiting
Phases
Unknown
Study type
Interventional
Source
ClinicalTrials.gov
Registry ID
NCT07472010
Acronym
MSE-BR
Enrollment
52
Registered
2026-03-16
Start date
2025-12-15
Completion date
2026-06-19
Last updated
2026-03-16

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Autonomic Nervous System, Muscle Tone, Psychological State

Keywords

Mandibular Stretching Exercise, 4-4-8 Breathing Exercise, Breathing Techniques, Autonomic Nervous System, Muscle Tone, Psychological State

Brief summary

Mandibular stretching exercises and breathing techniques may influence autonomic nervous system activity, muscle tone, and psychological state. This randomized controlled trial aims to investigate the effects of mandibular stretching exercise and the 4-4-8 breathing exercise on autonomic nervous system activity, muscle tone, and psychological parameters in healthy university students. Participants will be randomly assigned to intervention groups and evaluated before and after the intervention period.

Detailed description

The autonomic nervous system plays an important role in regulating physiological and psychological responses. Breathing techniques and mandibular stretching exercises are non-invasive approaches that may influence autonomic nervous system activity and muscle tone through neuromuscular and respiratory mechanisms. However, the combined or individual effects of these interventions on autonomic regulation and psychological outcomes remain insufficiently investigated. This randomized controlled trial aims to examine the effects of mandibular stretching exercise and the 4-4-8 breathing exercise on autonomic nervous system activity, muscle tone, and psychological state in healthy university students. Participants will be randomly allocated to intervention groups according to the study protocol. Baseline and post-intervention measurements will be collected to evaluate changes in autonomic nervous system parameters, muscle tone, and psychological status. The findings of this study may contribute to understanding the potential benefits of simple non-pharmacological interventions such as mandibular stretching and breathing exercises in improving physiological regulation and psychological well-being.

Interventions

OTHERMandibular Stretching Exercise

Participants perform mandibular stretching exercises according to the study protocol

OTHER4-4-8 Breathing Exercise

Participants perform the 4-4-8 breathing exercise according to the study protocol.

Sponsors

Mehin Mammadzada
Lead SponsorOTHER

Study design

Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
NONE

Intervention model description

Participants will be randomly assigned to intervention groups including mandibular stretching exercise, 4-4-8 breathing exercise, or control group. The effects of the interventions on autonomic nervous system activity, muscle tone, and psychological state will be evaluated.

Eligibility

Sex/Gender
ALL
Age
18 Years to 30 Years
Healthy volunteers
Yes

Inclusion criteria

* Inclusion Criteria: * Healthy university students * Age between 18 and 30 years * Voluntary participation in the study * Ability to understand and perform mandibular stretching exercise and 4-4-8 breathing exercise

Exclusion criteria

* History of neurological disorders * Musculoskeletal disorders affecting the jaw, neck, or respiratory system * Respiratory diseases * Use of medications that may affect the autonomic nervous system * Inability to perform the exercises required in the study

Design outcomes

Primary

MeasureTime frameDescription
Autonomic Nervous System ActivityBaseline and 20, 40, 80, and 120 minutes after the interventionAutonomic nervous system activity will be assessed by measuring heart rate (beats per minute) using a digital pulse oximeter and blood pressure (mmHg) using an automatic blood pressure monitor. Measurements will be recorded at baseline and repeatedly after the intervention period (20, 40, 80, and 120 minutes).

Secondary

MeasureTime frameDescription
Muscle ToneBaseline and 20, 40, 80, and 120 minutes after the interventionMuscle activity of the temporalis and masseter muscles will be assessed using surface electromyography (EMG). EMG amplitude (microvolts, µV) will be recorded at baseline and repeatedly after the intervention period (20, 40, 80, and 120 minutes).
Psychological StateBaseline and 20, 40, 80, and 120 minutes after the interventionPsychological state will be assessed using the State-Trait Anxiety Inventory (STAI) questionnaire. STAI scores (range 20-80 points) will be recorded at baseline and repeatedly after the intervention period (20, 40, 80, and 120 minutes).

Countries

Turkey (Türkiye)

Contacts

CONTACTMehin Mammadzada, MSc Student
mammadzadamehin01@gmail.com+905313124790
PRINCIPAL_INVESTIGATORNeslihan Altuntaş YILMAZ, PhD

Necmettin Erbakan University

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Mar 17, 2026