Healthy Participants
Conditions
Keywords
Inspiratory Muscle Warm-Up, Inspiratory Muscle Strength, Maximal Inspiratory Pressure, Respiratory Muscle Performance, Aerobic Exercise, Anaerobic Exercise
Brief summary
Inspiratory muscle warm-up (IMW) is a specific pre-exercise breathing intervention designed to prepare the respiratory muscles before physical activity and potentially enhance exercise performance. Previous research suggests that IMW may acutely improve inspiratory muscle function; however, the duration of these effects during exercise and their responses under different exercise intensities remain unclear. Furthermore, placebo-controlled comparisons are limited in the current literature. The purpose of this study is to investigate the acute effects of inspiratory muscle warm-up on inspiratory muscle strength during aerobic and anaerobic exercise in trained athletes. The study will compare three experimental conditions: a standardized inspiratory muscle warm-up protocol, a placebo breathing warm-up, and a control condition without respiratory warm-up. In this repeated-measures interventional study, trained athletes aged 14-30 years will complete three experimental sessions in randomized order. The active intervention consists of inspiratory muscle warm-up performed at 40% of maximal inspiratory pressure (MIP). The placebo condition includes a low-resistance breathing protocol designed to mimic the intervention without providing a meaningful training stimulus. The control condition involves exercise performed without respiratory warm-up. Participants will perform treadmill exercise at intensity levels representing aerobic and anaerobic workloads, defined according to percentage of maximal heart rate. Inspiratory muscle strength (MIP) will be assessed at baseline, immediately after the warm-up protocols, and periodically during exercise to evaluate time-dependent changes. The primary objective is to determine how long the acute increase in inspiratory muscle strength is maintained during exercise and to compare responses between aerobic and anaerobic athletes across the three conditions. The findings may provide evidence-based guidance for integrating respiratory muscle warm-up strategies into sport-specific preparation routines.
Detailed description
Inspiratory muscle warm-up (IMW) has emerged as a non-pharmacological pre-exercise strategy aimed at enhancing respiratory muscle readiness prior to physical activity. The inspiratory muscles, particularly the diaphragm and accessory respiratory muscles, play a critical role in meeting increased ventilatory demands during exercise. Acute respiratory muscle fatigue has been associated with reduced performance through mechanisms such as altered ventilatory efficiency and activation of the respiratory muscle metaboreflex, which may redistribute blood flow away from locomotor muscles. While previous studies have reported beneficial acute effects of IMW on respiratory and performance parameters, the temporal characteristics of these effects during ongoing exercise remain insufficiently understood. Existing research has primarily focused on pre- and post-intervention comparisons, often evaluating inspiratory muscle strength immediately following IMW without assessing how long these acute improvements persist during exercise. Furthermore, the influence of exercise intensity domains and athlete specialization (aerobic vs. anaerobic disciplines) on the duration of IMW-induced effects has not been systematically investigated. Another limitation of prior studies is the limited use of placebo-controlled designs, which are important for distinguishing physiological effects from expectancy or psychological influences associated with warm-up interventions. The present study is designed as a repeated-measures interventional investigation to evaluate the acute time course of inspiratory muscle strength responses following inspiratory muscle warm-up under different exercise conditions. Each participant will complete three experimental sessions, including: (1) an active inspiratory muscle warm-up protocol performed at a standardized relative intensity, (2) a placebo breathing warm-up designed to mimic the intervention without providing a meaningful physiological load, and (3) a control condition without respiratory warm-up. The order of conditions will be counterbalanced to minimize order effects. Exercise trials will involve treadmill running performed at predefined intensity domains representing aerobic and anaerobic workloads based on individual maximal heart rate. Inspiratory muscle strength will be measured repeatedly throughout exercise to identify the time-dependent decline relative to baseline and post-warm-up levels. This design allows examination of both immediate and sustained effects of respiratory muscle preparation strategies. By comparing responses between athletes from predominantly aerobic and anaerobic sports backgrounds, the study seeks to explore whether differences in energy system utilization, muscle fiber composition, and ventilatory demands influence the effectiveness and duration of inspiratory muscle warm-up. The findings may contribute to a more precise understanding of respiratory muscle preparation and support evidence-based integration of breathing-specific warm-up strategies into athletic training and competition routines.
Interventions
A standardized inspiratory muscle warm-up performed using a breathing resistance device set at 40% of maximal inspiratory pressure (MIP). Participants perform resisted inspiratory efforts prior to exercise to acutely activate respiratory muscles.
A sham breathing warm-up performed using the same device and breathing pattern as the active intervention but with resistance set at 15% of maximal inspiratory pressure (MIP), intended to minimize physiological loading while maintaining procedural similarity.
Sponsors
Study design
Masking description
Participants and study staff are blinded to whether the breathing warm-up is performed at 40% MIP (active IMW) or as a placebo/sham protocol. Warm-up loads are pre-set by an independent staff member not involved in testing, and conditions are labeled with neutral codes (e.g., A/B/C). The intervention administrator and outcome assessor record and analyze outcomes using coded conditions. Unblinding occurs only after data collection is completed. (If a no-warm-up control session is included, it is described separately and may not be fully blind unless a sham breathing routine is used.)
Intervention model description
Participants will complete three conditions (inspiratory muscle warm-up, placebo breathing warm-up, and control with no respiratory warm-up) in a randomized crossover design. All participants will undergo each condition in separate sessions, with the order counterbalanced and sessions separated by adequate washout periods to minimize carryover effects. Inspiratory muscle strength will be assessed repeatedly within each session to characterize the time course of acute responses during aerobic and anaerobic exercise workloads.
Eligibility
Inclusion criteria
* Age between 14 and 30 years. * Regular participation in organized sports training for at least 4 years. * Engaged in predominantly aerobic or anaerobic sports disciplines. * Training frequency of at least 3 sessions per week. * No known cardiopulmonary disease affecting respiratory function. * Ability to safely perform maximal effort treadmill exercise. * No respiratory illness within the last 12 weeks. * Willingness to comply with study procedures. * Provision of written informed consent (and parental consent for minors where applicable).
Exclusion criteria
* History of asthma, chronic obstructive pulmonary disease (COPD), or other respiratory disorders. * Age below 14 years or above 30 years. * Orthopedic injury or condition limiting running ability. * Participation in inspiratory muscle training within the last 12 months. * Use of medications affecting respiratory or cardiovascular function. * Active smoking. * Failure to provide informed consent.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Time to Decline in Maximal Inspiratory Pressure (MIP) During Exercise | From exercise onset until the time of MIP decline during a single acute exercise testing session (up to 30 minutes). | Time (minutes) until maximal inspiratory pressure (MIP) decreases below baseline or post-warm-up levels during aerobic and anaerobic exercise following inspiratory muscle warm-up, placebo breathing warm-up, or control conditions. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Acute Change in Maximal Inspiratory Pressure After Warm-Up | From exercise onset until MIP declines below baseline or post-warm-up values, assessed at minutes 2, 4, 6, 8, and 10, then at minutes 14, 18, 22, 26, and 30 (up to 30 minutes). | Difference between baseline MIP and immediate post-warm-up MIP across experimental conditions. |
| Repeated MIP Measurements During Exercise | Every 2 minutes during exercise. | Periodic maximal inspiratory pressure values measured at predefined intervals during treadmill exercise. |
Countries
Turkey (Türkiye)