Periapical Periodontitis
Conditions
Brief summary
Evaluate and compare the disinfecting efficacy of two supplementary antimicrobial protocols in teeth with apical periodontitis
Detailed description
This study aims to evaluate and compare the disinfecting efficacy of two supplementary antimicrobial protocols in teeth with apical periodontitis: Er,Cr:YSGG laser activated irrigation and ultrasonic activation using a piezoelectric ultrasonic device (Dentsply International Inc., York, PA, USA) and Irrisafe tip (Satelec/Acteon, Merignac, France), comparing the detection of bacteria before and after instrumentation. Secondary aims are to assess presence of bacteria before and after chemomechanical preparation using two microbiological methods: * ATP level analysis using the Endocator device * Quantitative PCR (qPCR) for total bacterial quantification.
Interventions
DEVICEDesinfection device
Desinfection protocol with (LAI): Er,Cr:YSGG laser (2780 nm) delivered with a radial-firing tip.
DEVICEAnalytical microbiological methods
Endocator: Measures ATP levels (Endoscore and RLU) to rapidly assess bacterial load in less than one minute.
Sponsors
State University of New York at Buffalo
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
NONE
Intervention model description
This is a parallel-group interventional study in which participants are allocated to different treatment arms according to the intervention received (laser or ultrasound), following standardized clinical protocols.
Eligibility
Sex/Gender
ALL
Age
18 Years to 70 Years
Healthy volunteers
Yes
Inclusion criteria
* Adult patients able to give informed consent and willing/able to comply with study procedures and follow-up. * Molar teeth indicated for endodontic treatment. * Teeth with intact pulp-chamber walls (no extensive coronal destruction). * Necrotic pulp confirmed by pulp testing. * Clinical and radiographic evidence of asymptomatic apical periodontitis.
Exclusion criteria
* Immature teeth * Teeth with calcified or obliterated canals * Teeth with extensive coronal destruction * Teeth with previous endodontic treatment * Symptomatic apical periodontitis * Teeth with periodontal pockets greater than 4 mm * Patients who have received systemic antibiotics in the last 3 months * Patients with a history of diabetes or immune impairment * Pregnant patients
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Reduction of bacteria in the root canal system | Immediately after the irrigation activation procedure, ATP levels will be measured, and qPCR analysis will be performed at the end of the study, once all samples have been collected. | Evaluation of the effectiveness of different irrigation activation devices by assessing bacterial reduction in the root canal system. ATP levels will be measured (Endoscore and RLU values) to provide a rapid assessment of bacterial load in less than one minute. Subsequently, bacterial reduction will be confirmed by quantitative polymerase chain reaction (qPCR) analysis |
Countries
United States
Contacts
CONTACTStephen Lyon, DDS
CONTACTThamyres Monteiro, MSc
Outcome results
None listed