Inactivity, Physical
Conditions
Keywords
Exercise, Metabolism
Brief summary
Exercise can reduce the risk of several chronic diseases. However, recent studies have suggested sitting for too long before exercising can decrease exercise benefits. This study will help determine why this may occur.
Detailed description
Previous work demonstrated a greater utilization of carbohydrates (CHO) at the end of aerobic exercise with a day of reduced activity (\<5,000 steps). Additionally, there are differences in the RNA profile at rest and during exercise in pathways related to inflammation, metabolism, and innate immunity that warrant further research. Together, these data highlight that a single day of reduced activity alters systemic homeostasis that affects the exercise response. Given the potency of exercise to ameliorate risk of cardiovascular disease, more research is needed to find factors to augment the exercise response. Therefore, this study has two goals: 1) Analyze circulating metabolomics, and 2) metabolomics and proteomics in response to exercise after a day of reduced activity.
Interventions
BEHAVIORALDecreased step count
Subjects will walk less than 5,000 steps
Sponsors
Concordia University Wisconsin
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
PREVENTION
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 30 Years
Healthy volunteers
Yes
Inclusion criteria
* • between the ages of 18-30 years old
Exclusion criteria
* • free from acute or chronic illness (cardiac, pulmonary, liver, or kidney abnormalities, cancer, uncontrolled hypertension, insulin- or non-insulin dependent diabetes or other known metabolic disorders) * do not take medications related to metabolism (i.e., statins, metformin, GLP-1 agonists) * free from orthopedic limitations (including any artificial joints) * currently smoke or participate in other forms of tobacco use.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Concentration of metabolites within the plasma | Participants will complete the study within 6 weeks of enrollment. | We will collect blood before and immediately after exercise. Plasma will be separated and sent for untargeted metabolomic analysis. |
| Concentration of proteins within the plasma | Participants will complete the study within 6 weeks of enrollment. | We will collect blood before and immediately after exercise. Plasma will be separated and sent for untargeted proteomic analysis. |
Countries
United States
Contacts
CONTACTKevin J Gries, PhD
Outcome results
None listed