Abdominal Surgery
Conditions
Keywords
Microbiome, intestinal anastomosis, dehiscence
Brief summary
Recent research suggests a significant link between gut microbiota and anastomotic leakage (AL) in colorectal surgery. Patients who develop AL have a higher abundance of bacteria from the Lachnospiraceae family and lower microbial diversity. Considering the bibliographic data, our main interest is to analyze the microbial population of patients in our social environment and then look for differences in the microbiome between those who have suffered an anastomotic dehiscence (around 8-9% according to the results of our hospital's Surgery Department) and those who have not.
Detailed description
The study is designed as a prospective, descriptive, non-interventional research project in which some variables will be analyzed transversally. Patients will be selected by the clinical members of the research team from within the population in our setting who will undergo abdominal surgery involving colorectal intestinal anastomosis. Once accepted, stool samples will be requested prior to surgical preparation, during surgery, and afterwards to determine their microbiome; in this way, we will attempt to correlate the evolution of their anastomosis with their intestinal bacterial population.
Interventions
OTHERNot applicable- observational study
Fecal sample collection
Sponsors
Instituto de Investigación Sanitaria de la Fundación Jiménez Díaz
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Patients who have undergone colorectal intestinal anastomosis. * Signing of the informed consent form. * Age ≥18 years with good functional status. * Availability for postoperative follow-up.
Exclusion criteria
* Emergency surgery. * Intra-abdominal sepsis or previous dehiscence. * Severe immunosuppression. * Use of antibiotics in the 3 months prior to preoperative preparation. * Pregnancy or breastfeeding.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Understand the patient's microbiome | From enrollment to a month post surgery | To understand the microbiome of patients undergoing colorectal surgery and the effect of current surgical preparation on the microbiota in our local population. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Predictive indicators of colorectal anastomosis postsurgery | From surgery to a month | Predictive indicators of postoperative outcomes in patients undergoing colorectal anastomosis surgery through metagenomic characterization of the gut microbiota. |
Countries
Spain
Contacts
CONTACTHéctor Guadalajara-Labajo, MD, PhD
PRINCIPAL_INVESTIGATORMariano García-Arranz, PhD
Health Research Institute Fundación Jiménez Díaz
Outcome results
None listed