Neuroinflammatory Biomarkers After General Anesthesia: A Comparison Study

Evaluation of Preoperative and Postoperative Serum BDNF, IL-1β, and HMGB1 Levels and Cognitive Changes in Patients Undergoing General Anesthesia: Differences Between Patients With No Prior General Anesthesia and Those With Multiple Anesthesia Exposures

Status

Not yet recruiting

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT07388160

Enrollment

160

Registered

2026-02-04

Start date

2026-01-25

Completion date

2026-09-25

Last updated

2026-02-04

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Anesthesia, Cognitive Disorders, Neuroinflammation

Keywords

Brain Derived Neurotrophic Factor, Interleukin-1 beta, General Anesthesia, Neuroinflammation, Cognitive Dysfunction, Biomarkers, Sevofluran, Inflammatory Cytokines, Anesthesia Neurotoxicity

Brief summary

This prospective, observational study investigates the impact of repeated general anesthesia exposure on neuroinflammatory biomarkers and neurotrophic factors. The study will enroll 160 adult patients (aged 18-65 years) scheduled for elective cholecystectomy surgery, divided into two groups: patients with no previous general anesthesia exposure (n=80) and patients with at least one or more previous general anesthesia exposures (n=80). Serum levels of Brain-Derived Neurotrophic Factor (BDNF), Interleukin-1 beta (IL-1β), and High Mobility Group Box 1 (HMGB1) will be measured using ELISA method at two time points: preoperatively (baseline) and 24 hours postoperatively. Cognitive function will also be assessed at both time points. The primary objective is to evaluate whether multiple exposures to general anesthesia lead to significant differences in serum BDNF, IL-1β, and HMGB1 levels, reflecting changes in neurotrophic balance and neuroinflammatory response. This study aims to provide insights into the potential biochemical mechanisms underlying anesthesia-related cognitive changes and contribute original clinical data to the current literature on general anesthesia safety and neurobiological effects.

Interventions

None listed

Study design

Observational model

CASE_CONTROL

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

18 Years to 65 Years

Healthy volunteers

Yes

Inclusion criteria

* Age 18-65 years * Scheduled for elective cholecystectomy surgery * Either no previous general anesthesia exposure OR at least one or more previous general anesthesia exposures * Ability to provide informed consent * American Society of Anesthesiologists (ASA) physical status I-II

Exclusion criteria

* Chronic neurological diseases (Alzheimer's disease, Parkinson's disease, multiple sclerosis, etc.) * Psychiatric illness requiring medication * Metabolic syndrome (diabetes mellitus, hypertension, cardiac disease) * Chronic inflammatory diseases (rheumatoid arthritis, systemic lupus erythematosus, etc.) * Corticosteroid or immunosuppressive therapy within the last 6 months * Patients requiring intensive care admission * Pregnancy or breastfeeding * Known allergy to anesthetic agents * Inability to provide informed consent

Design outcomes

Primary

Measure	Time frame	Description
Change in Serum BDNF Levels	Preoperative (baseline) and 24 hours postoperative	Change in serum Brain-Derived Neurotrophic Factor (BDNF) concentration measured by ELISA method, comparing preoperative baseline levels with postoperative levels at 24 hours
Change in Serum HMGB1 Levels	Preoperative (baseline) and 24 hours postoperative	Change in serum High Mobility Group Box 1 (HMGB1) concentration measured by ELISA method, comparing preoperative baseline levels with postoperative levels at 24 hours
Change in Serum IL-1β Levels	Preoperative (baseline) and 24 hours postoperative	Change in serum Interleukin-1 beta (IL-1β) concentration measured by ELISA method, comparing preoperative baseline levels with postoperative levels at 24 hours

Secondary

Measure	Time frame	Description
Correlation Between Biomarkers	At 24 hours postoperative	Analysis of correlations between serum BDNF, IL-1β, and HMGB1 levels to evaluate the relationship between neurotrophic balance and systemic inflammatory response following general anesthesia
Comparison Between Single vs Multiple Anesthesia Exposure Groups	Preoperative (baseline) and 24 hours postoperative	Statistical comparison of serum BDNF, IL-1β, and HMGB1 levels between patients with no previous general anesthesia exposure and those with previous general anesthesia exposure(s)

Contacts

CONTACTsevgi kutlusoy, Assoc. Prof. Dr.

stopal70@hotmail.com+905449696144

STUDY_CHAIRsevgi kutlusoy, Assoc. Prof. Dr.

Saglik Bilimleri Universitesi

Outcome results