Gastritis, Periodontal Diseases
Conditions
Keywords
gastritis, microbiome, metagenomic analysis, dysbiosis
Brief summary
The aim of this study to evaluate the microbial profile in gastritis and healthy patients in different periodontal conditions via next generation technologies.
Detailed description
This study aims to determine the density of bacteria associated with periodontal disease and to evaluate oral dysbiosis in gastritis patients using metagenomic analysis.
Interventions
OTHERsaliva collection
collecting unstimulated saliva
Sponsors
Aydin Adnan Menderes University
Study design
Observational model
COHORT
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
20 Years to 55 Years
Healthy volunteers
Yes
Inclusion criteria
* no systemic diseases other than gastritis non-smoker
Exclusion criteria
* Participants who took regular medications that could affect of periodontal tissue or oral microbiome pregnancy or lactation Patients who received periodontal treatment within the last 6 months
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Salivary microbial profile | 1 month | To compare the oral flora profiles of the study groups, a microbiome-based next-generation sequencing approach targeting the 16S rRNA gene was employed. In this metagenomic study, following DNA extraction, phylogenetic classification was performed using the V3-V4 hypervariable regions of the 16S rRNA gene. Using the obtained data, total bacterial diversity and density were compared through alpha and beta diversity analyses. Bacterial taxa that differed between the groups were identified using LEfSe analysis. |
Countries
Turkey (Türkiye)
Outcome results
None listed