Antibiotic Sensitivity Testing of Different Antibiotics According to Clsi 25, Detection of Resistance Gene in Isolates Using Conventional Pcr, Detection of Some Virulence Genes Using Conventional Pcr, Effect of Ceftazidime-Avibactam on Carbapenemase-Producing Klebsiella Pneumoniae, ISOLATION OF KLEB. by Culture on macConkey Agar
Conditions
Brief summary
Specimen Collection : Clinical specimens included blood, urine, bronchoalveolar lavage fluid, wound, peritoneal fluid, and tracheal aspirate obtained from patients with suspected bacterial infections collected in dry sterile well-closed plastic cups. Bacterial identification by Gram stain, culture and biochemical rections * analysisThe resistance pattern of the isolates will be detected by disc diffusion method. * phenotypic detection of carbapenemases. • Detection of carbapenem resistance genes (KPC, VIM, IMP, OXA-48, NDM) by conventional PCR.
Detailed description
Carbapenem-resistant Enterobacterales (CRE) poses an urgent global public health threat, with more than 1,100 documented deaths, as reported in a 2019 antibiotic resistance publication by the U.S. Centers for Disease Control and Prevention. Carbapenem-resistant Enterobacterales, including carbapenem-resistant Klebsiella pneumoniae (CRKP), is associated with higher mortality compared with infections caused by carbapenem-susceptible Enterobacterales infections Specimen Collection : Clinical specimens included blood, urine, bronchoalveolar lavage fluid, wound, peritoneal fluid, and tracheal aspirate obtained from patients with suspected bacterial infections collected in dry sterile well-closed plastic cups. Bacterial identification by Gram stain, culture and biochemical rections * analysisThe resistance pattern of the isolates will be detected by disc diffusion method. * phenotypic detection of carbapenemases. • Detection of carbapenem resistance genes (KPC, VIM, IMP, OXA-48, NDM) by conventional PCR.
Interventions
GENETIC. - Genotypic detection of carbapenemase genes by conventional PCR.
Specimen Collection : Clinical specimens included blood, urine, bronchoalveolar lavage fluid, wound, peritoneal fluid, and tracheal aspirate obtained from patients with suspected bacterial infections collected in dry sterile well-closed plastic cups. Bacterial identification by Gram stain, culture and biochemical rections - analysisThe resistance pattern of the isolates will be detected by disc diffusion method. - phenotypic detection of carbapenemases. • Detection of carbapenem resistance genes (KPC, VIM, IMP, OXA-48, NDM) by conventional PCR.
Sponsors
Sohag University
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Healthy volunteers
Yes
Inclusion criteria
Infections caused by klebsiella pneumoniae like skin infections, chest infections, surgical site infections, and urinary tract Infections.-
Exclusion criteria
* Infections caused by any organisms other than klebsiella pneumoniae and carbapenem resistant klebsiella pneumoniae by mechanism other than carbapenemases production.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| 1 ISOLATION OF KLEB. by culture on macConkey agar | 6 months from January to may |
| Antibiotic sensitivity testing of different antibiotics according to clsi 25 | 6 months from January to may |
| Effect of Ceftazidime-Avibactam on Carbapenemase-Producing Klebsiella Pneumoniae | 6 months from January to may |
| Detection of resistance gene in isolates using conventional pcr | 6 months from June to December |
| Detection of some virulence genes using conventional pcr | 6 months from June to December |
Countries
Egypt
Contacts
CONTACTWalaa Ismael, Demonstrator
Outcome results
None listed