Effects of Propolis Flavonoids on TLR4 Signaling and Inflammation in Human PDLSCs In Vitro

Effect of Propolis-Derived Flavonoid Extract on TLR4 Signaling and Inflammatory Cytokine Expression in Human Periodontal Ligament Stem Cells (PDLSCs): An In-Vitro Study

Status

Not yet recruiting

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT07367451

Enrollment

Registered

2026-01-26

Start date

2026-01-25

Completion date

2026-02-10

Last updated

2026-02-05

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Inflammatory Cytokine Expression

Keywords

TLR4, flavonoid-rich propolis, Periodontal ligament, stem cells

Brief summary

This in-vitro study evaluated the effects of a flavonoid-rich propolis extract on Toll-like receptor 4 (TLR4) signaling and inflammatory cytokine expression in human periodontal ligament stem cells (PDLSCs). PDLSCs were treated with propolis flavonoids under baseline conditions and following lipopolysaccharide (LPS)-induced inflammation, with untreated cells serving as controls. TLR4 gene expression was analyzed using PCR, while levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) were quantified by ELISA. Cell viability and cytotoxicity at different extract concentrations were assessed using the MTT assay. The study aimed to determine the immunomodulatory and cytocompatible potential of propolis flavonoids in regulating inflammatory responses in PDLSCs.

Interventions

DRUGFlavonoid extract from propolis

Preparation of Propolis Flavonoid Extract Concentrations for testing: 5 µg/mL 10 µg/mL 20 µg/mL

OTHERno medication added

No medication will be added to the second group

Study design

Observational model

OTHER

Time perspective

CROSS_SECTIONAL

Eligibility

Sex/Gender

ALL

Healthy volunteers

Inclusion criteria

* invitro study on Human Periodontal Ligament Stem Cells

Exclusion criteria

* none

Design outcomes

Primary

Measure	Time frame	Description
TLR4 expression	48 hours	To evaluate the expression of TLR4 in PDLSCs after treatment with propolis flavonoid extract.

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 6, 2026