Hip Osteoarthritis, Knee Osteoarthristis, Total Hip Arthroplasty (THA), Total Knee Arthroplasty (TKA)
Conditions
Keywords
Total Knee Replacement, Total Hip Replacement, Trace Elements, Osteoarthritis, Cytokines, Oxidative Stress
Brief summary
The aim of this observational study is to determine content of rare metals and other elements, along with the levels of selected immunological parameters, in the tissue of the hip or knee joint in the course of degenerative diseases. Determining potential disturbances in the levels or functions of these parameters may in the future contribute to improving treatment - for example, through supplementation of deficient metals or modulation at the immunological level. The main question it aims to answer is: Are selected rare metals, trace elements and other selected immunological parameters lowered in the tissue of the hip or knee joint as well as in serum in the course of osteoarthritis?
Interventions
None listed
Sponsors
Medical University of Warsaw
Study design
Observational model
OTHER
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* 18 years old and more * patient qualified for primary TKA or primary THA * patient diagnosed with hip or knee osteoarthritis * a complete set of tissues of good quality and volume is available * does not meet the
Exclusion criteria
.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Enzymatic activity of selected proteolytic enzymes in periarticular tissue homogenate (e.g., metalloproteinases and selected serine/cysteine proteases). | Perioperative/Periprocedural (during primary THA/TKA; index hospitalization) | Enzymatic activity assessed using biochemical/spectrofluorometric methods and normalized to total protein. Each enzyme will be reported separately. Data is planned to be reported as activity units per mg protein (e.g. U/mg protein). |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Protein concentration of selected inflammatory markers in blood (e.g., TNF-α, IL 17α). | Perioperative/Periprocedural (day of surgery; index hospitalization for primary THA/TKA) | Protein concentrations measured by enzyme immunoassays (EIA/ELISA). Each protein will be reported separately as pg/mL. |
| Trace element concentration in periarticular tissues (e.g., Zn, Cu, Fe). | Perioperative/Periprocedural (day of surgery; index hospitalization for primary THA/TKA) | Trace element content measured by Inductively Coupled Plasma - Mass Spectrometry (ICP-MS). Each element will be reported separately. The units for serum measurements is µg/L, and for other tissue is mg/kg dry weight. |
| Relative mRNA expression of selected inflammatory and bone metabolism markers in periarticular tissue (e.g. TNF-α, RANK, RANKL, BMP-12, IL-17α, IL-17αR). | Perioperative/Periprocedural (day of surgery; index hospitalization for primary THA/TKA) | Relative mRNA expression measured by RT-qPCR and reported as fold change (e.g., 2\^-ΔΔCt). Each gene will be reported separately (no aggregation into a single score). |
Countries
Poland
Contacts
CONTACTArtur Stolarczyk, Professor
Outcome results
None listed