Circadian Dysregulation, Circadian Misalignment, Circadian Rhythm, Insufficient Sleep, Lifestyle Factors, Light Exposure, Metabolism Changes, Sleep, Sleep Deprivation, Sleep Hygiene, Sleep Hygiene, Inadequate
Conditions
Keywords
Sleep, Light Exposure, Metabolism, Circadian Rhythm, Food Intake, Lifestyle Factors, Sleep Hygiene
Brief summary
Sleep is an important factor for overall health. This study will see how different light exposure patterns and food intake impact a person's metabolism (how the body breaks down food) when sleeping is reduced. Participants will attend 6 to 8 in-person visits to the study clinic, including three overnight stays. People will complete surveys and medical tests. The study will last about 4 to 6 months.
Detailed description
This study is a randomized in-lab, cross-over trial. Each participant will complete all three arms in either condition order #1 (A-B-C) or order #2 (A-C-B). Each arm will consist of 5 nights of experimental sleep restriction followed by a constant routine protocol for assessment of 24-h rhythms. Sleep restriction in the three arms will occur under the following conditions: (A-control condition) Sleep Restriction with Central and Peripheral Alignment; (B) Sleep Restriction with Central Clock Misalignment; and (C) Sleep Restriction with Peripheral Misalignment. Prior to enrollment participants will complete a comprehensive medical history and clinical overnight sleep disorders screening. Baseline consists of a \ 2-week ambulatory real-world monitoring segment that will occur immediately prior to each in-lab sleep restriction condition. Following the 5 days of sleep restriction in each arm, participants will complete an intravenous glucose tolerance test to analyze insulin sensitivity, prior to completing the constant routine.
Interventions
BEHAVIORALCentral Clock Misalignment
Light exposure will be dimmed during the first 4 hours of scheduled wakefulness, with bright light exposure during the nighttime hours of extended wakefulness.
BEHAVIORALPeripheral Misalignment
Most daily calories will be given later in the day to shift eating patterns toward the nighttime hours.
Sponsors
University of Utah
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
TRIPLE (Subject, Investigator, Outcomes Assessor)
Masking description
Randomization to condition order will be concealed to research staff and participants until the conclusion of the condition "A", which all participants will complete first.
Eligibility
Sex/Gender
ALL
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
1. Age: 18-35 years old; equal numbers of men and women 2. Body Mass Index (BMI): 18.5-24.9 kg/m2 3. Sleep Habits: habitual self-reported average total sleep time (TST) 7-9 hours per night for prior 6 months
Exclusion criteria
1. Clinically diagnosed sleep disorder or apnea hypopnea index (AHI) ≥5 2. Evidence of significant organ system dysfunction or disease (e.g., heart disease, diabetes) 3. Fasting plasma glucose ≥100 mg/dL 4. Major psychiatric illness (e.g., major depressive disorder) 5. Cancer that has been in remission less than 5 years 6. History of shift-work in prior year 7. Weight change \>5% of body weight over prior six months 8. Currently following a weight-loss program 9. Menopause 10. Pregnant/nursing 11. Greater than 5-day variation in menstrual cycle length month-to-month 12. Currently smoking 13. Alcohol intake \>14 drinks/week or \>3 drinks/day. 14. Use of prescription medications (except oral contraceptives) within one month prior to or during in-lab visits. 15. Consumption of illegal drugs or \>500mg per day of caffeine.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Insulin Sensitivity | Immediately following the 5-day sleep restriction segment | Insulin sensitivity will be measured by intravenous glucose tolerance tests. |
| Internal Circadian Misalignment (Uridine and Isobutyryl-L-carnitine) | During the constant routine, following the 5-day sleep restriction segment | Phase angle between DLMO and acrophase of uridine and isobutyryl-L-carnitine during constant routine. |
Countries
United States
Contacts
CONTACTVictoria Miranda, MS
CONTACTGrace Zimmerman, PhD
PRINCIPAL_INVESTIGATORChristopher Depner, PhD
University of Utah
Outcome results
None listed