Liver Cancer
Conditions
Keywords
liver cancer
Brief summary
This prospective, single-center observational study evaluates the diagnostic accuracy of a novel Folate-targeted Near-Infrared II Carbon Dot (FA-CDots) probe for the ex vivo assessment of Hepatocellular Carcinoma (HCC). Following standard radical hepatectomy, resected liver specimens will be incubated with the FA-CDots probe and imaged using a NIR-II fluorescence system. The study aims to determine the feasibility and accuracy of this technology in identifying tumor margins and micro-lesions by comparing the fluorescence imaging results with standard histopathological examination.
Interventions
DIAGNOSTIC_TESTEx Vivo FA-CDots Staining
The resected liver specimens are incubated with Folate-targeted Near-Infrared II Carbon Dots (FA-CDots) and imaged using a NIR-II fluorescence imaging system to identify tumor margins and micro-lesions. The results are compared with standard histopathology.
Sponsors
West China Hospital
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 80 Years
Healthy volunteers
No
Inclusion criteria
1. Primary diagnosis of Hepatocellular Carcinoma (HCC) confirmed clinically or by preoperative biopsy. 2. Scheduled for radical hepatectomy. 3. Preoperative imaging (CT/MRI) suggests indistinct tumor boundaries, infiltrative growth, or proximity to major vessels (expected margin \< 1cm). 4. Liver function classified as Child-Pugh Grade A. 5. ASA Physical Status classification I-III. 6. Participant or legal guardian willing to sign informed consent for the use of ex vivo tissues.
Exclusion criteria
1. Concomitant other malignant tumors. 2. Severe dysfunction of heart, lung, kidney, or brain unable to tolerate surgery. 3. Recurrent HCC. 4. Preoperative evidence of extrahepatic metastasis or major vascular invasion. 5. Received preoperative anti-tumor therapy. 6. Pregnant or lactating women.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Diagnostic Performance of FA-CDots (Sensitivity, Specificity, and AUC) | Immediate ex vivo analysis post-surgery | Evaluate the accuracy of FA-CDots in distinguishing HCC tissue from normal liver tissue. Metric: Area Under the Receiver Operating Characteristic Curve (AUC), Sensitivity, and Specificity. Calculation: Based on the Signal-to-Background Ratio (SBR) of the fluorescence images. A Positive result is defined as an SBR \> 2.0 (threshold to be optimized). Gold Standard: The diagnosis is verified by standard histopathological examination (H&E staining) of the corresponding tissue sites. |
Countries
China
Contacts
Primary ContactJiwei Huang Professor
Outcome results
None listed