Reversible Pulpitis, Irreversible Pulpitis
Conditions
Brief summary
intervention eligible patients with teeth diagnosed with reversible or irreversible pulpits will go for vital pulp therapy and the other group will be patients will go for extraction of caries free teeth for orthodontic reasons, blood samples will be collected from both groups to analyze the level of cytokines IL-8 and IL-10 and it's relation with the success of the pulp therapy
Interventions
PROCEDUREVital pulp therapy
1. Full medical and dental history will be taken from each patient. 2. Clinical and radiographic examination (Cone beam computed tomography) for the offending tooth. 3. After anesthesia, Rubber dam is applied and complete caries removal will be done, deeply inside the cavity, the excavation will be done using low speed hand piece and excavator. 4. A blood sample will be taken from the bleeding pulp tissue on a cotton pellet for Molecular test. 5. After exposure, the pulp will be amputated to the level of the canal orifices (full pulpotomy) pulp vitality was confirmed by the presence of bleeding pulp tissue from all canals. 6. Putty MTA will gently place over the pulp. 7. The cavity will be sealed with initial deep layer of resin modified glass ionomer restoration (RMGI) and final superficial layer of composite restoration.
PROCEDUREExtraction
1. Full medical and dental history will be taken from each patient. 2. Clinical and radiographic examination (Cone beam computed tomography) for the offending tooth. 3. After anesthesia, Rubber dam is applied. 4. A blood sample will be taken from the bleeding pulp tissue on a cotton pellet for Molecular test. 5. Extraction for orthodontic reasons.
PROCEDUREblood sample collection
after access opening blood sample will be collected
Sponsors
Mansoura University
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
16 Years to 45 Years
Healthy volunteers
No
Inclusion criteria
* The age ranges from 16-45 years medically free. * Tooth should be vital on cold testing. * Vital permanent premolar and molar on cold testing, with deep caries, subsequent pulp exposure and bleeding pulp tissue in all canals after complete pulpotomy. * Diagnosis is either reversible or irreversible pulpitis with/ without periapical rarefaction. * The tooth is restorable and free from advanced periodontal disease. * Soft tissues around the tooth are normal with no swelling or sinus tract.
Exclusion criteria
* Patients over 45 years of age. * Patients with clinical symptoms of periradicular inflammation or any periradicular radiographic lesions. * Negative results for cold and electrical pulp testing
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Clinical success | one day, one, four and twelve weeks. | post operative pain on cold testing binary outcome (Yes or no) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Radiographic success | Pre-operative , and after six months | Cone Beam Computed Tomography CBCT radiographic Examination for any periodontal widening or any periapical radiolucency. |
| level of cytokines | through study completion, an average of 6 months | level of Interleukin 8 and Interleukin 10 will be measured using Enzyme- Linked Immunosorbent assay (ELISA) Kit |
Countries
Egypt
Outcome results
None listed