Degenrative Disc Disease
Conditions
Brief summary
The general objective of this project is to define the transcriptional profile of primary human cells derived from the nucleus pulposus (NP), annulus fibrosus (AF), and cartilaginous endplate (CEP), and to use this information as a reference to assess the biological relevance of a biomimetic spinal unit model obtained through bioprinting. By developing an in vitro model of human origin that incorporates key components of the spinal unit and applying transcriptional analyses to both native cells and their counterparts recovered from the 3D construct, the study will evaluate how accurately the bioprinted model reproduces the identity and heterogeneity of the native discal environment.
Interventions
None listed
Sponsors
I.R.C.C.S Ospedale Galeazzi-Sant'Ambrogio
Study design
Observational model
OTHER
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
30 Years to 70 Years
Healthy volunteers
No
Inclusion criteria
* Signature of the Informed Consent for the study * Age 30-70 years (included) * Pfirrmann grade III-V * Need to undergo spinal surgery
Exclusion criteria
* Presence of HIV, HBC, HCV or TPHA infection
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Discovery of Tissue-Specific Molecular Markers in Intervertebral Disc Cellular Populations | From enrollment to data analysis (24 months) | To identify genes that are specifically upregulated in one of the three intervertebral disc cellular populations-nucleus pulposus (NP), annulus fibrosus (AF), and cartilaginous endplate (CEP)-through comparative gene expression analysis of paired samples obtained from the same donors. This aim seeks to determine tissue-specific molecular markers that will be subsequently applied to the characterization of bioprinted intervertebral disc models. |
Countries
Italy
Outcome results
None listed