Health Adults
Conditions
Keywords
Exercise, Bone turnover, Extracellular vesicles, Wnt/β-catenin signaling pathway
Brief summary
The goal of this study is to investigate the acute effects of drop-landing exercise on the bone metabolism biomarkers and extracellular vesicles in healthy young males, in order to gain a deeper understanding of the mechanotransduction mechanisms involved in bone metabolism. The main question aims to answer: • Does a single bout acute drop-landing exercise change serum sclerostin, other bone signaling markers, and circulating extracellular vesicles levels? Participants complete both the drop-landing and control trials in a randomized order, with a minimum washout period of one week between trials. During each trial, blood samples are collected at three time points: pre-, immediately post, and 1-hour post drop-landing/control exercise.
Interventions
BEHAVIORALExercise
During the drop-landing trial, participants perform drop-landing exercises from a block calibrated to generate approximately four times their body weight in vertical ground reaction force upon landing, employing a stiff-landing technique. Each repetition is separated by a 30-second interval, and 10 repetitions are performed as 1 set, totalling 6 sets. There is a 2-minute rest break between each set, during which the participants are asked to sit on a chair. The entire drop-landing exercise is recorded on the force plate. During the control trial, participants are required to walk up and down from the same height block without the requirement to perform the drop-landing movement. The number of repetitions, intervals, and rest breaks are completely consistent with the drop-landing exercise.
Sponsors
Loughborough University
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* 18-35 years old * Healthy * Male * Body mass index (BMI) of 18.5-29.9 * Can understand and follow trial instructions
Exclusion criteria
* Have any uncontrolled cardiovascular or respiratory disease * Have severe musculoskeletal or neurological disease likely to affect ability to perform an exercise * Have medical conditions known to affect musculoskeletal health * Have medications known to affect bone metabolism (e.g., corticosteroids, thyroxine, antiepileptic, and antiretroviral agents) * Regular (more than once per week) participation in high-intensity resistance or impact-type exercise in the past 6 months * Current smoker or vaper
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Serum sclerostin concentration | Blood samples are collected at pre- (baseline), immediately post, 1-hour post drop-landing/control exercise in main trials. | Serum sclerostin concentration is quantified using an enzyme-linked immunosorbent assay (ELISA) and reported in picograms per milliliter (pg/mL). |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Serum dickkopf Wnt signaling pathway inhibitor 1 (DKK1) concentration | Blood samples are collected at pre- (baseline), immediately post, 1-hour post drop-landing/control exercise in main trials. | Serum DKK1 concentration is quantified using an enzyme-linked immunosorbent assay (ELISA) and reported in picograms per milliliter (pg/mL). |
| Serum osteoprotegerin (OPG) concentration | Blood samples are collected at pre- (baseline), immediately post, 1-hour post drop-landing/control exercise in main trials. | Serum OPG concentration is quantified using an enzyme-linked immunosorbent assay (ELISA) and reported in picograms per milliliter (pg/mL). |
| Serum irisin concentration | Blood samples are collected at pre- (baseline), immediately post, 1-hour post drop-landing/control exercise in main trials. | Serum irisin concentration is quantified using an enzyme-linked immunosorbent assay (ELISA) and reported in picograms per milliliter (pg/mL). |
| Circulating extracellular vesicle (EV) particle concentration and size | Blood samples are collected at pre- (baseline), immediately post, 1-hour post drop-landing/control exercise in main trials. | EVs are isolated from plasma using ultracentrifugation. EV particle concentration is quantified by nanoparticle tracking analysis (NTA) and reported as particles per milliliter (particles/mL). EV size is also assessed by NTA and reported in nanometers (nm). |
| Ground reaction force during drop-landing exercise | Ground reaction force is recorded during each drop-landing exercise. | Ground reaction force is recorded in newtons (N) during each drop-landing exercise using a force plate in the drop-landing trial. Ground reaction force is reported in multiples of body weight (×BW). |
Countries
United Kingdom
Outcome results
None listed