Endothelial Dysfunction, Type 2 Diabetes (T2DM)
Conditions
Brief summary
Exaggerated blood pressure responses to exercise in individuals with Type 2 Diabetes significantly increase the risk of heart attack, stroke, and cardiovascular death, while also limiting exercise capacity and therapeutic benefits of physical activity. This research will determine whether impaired blood vessel function and excessive cellular damage from oxygen-containing molecules cause these dangerous blood pressure responses during exercise. The findings will establish whether targeting cellular antioxidant systems represents a new therapeutic approach to improve exercise tolerance and reduce cardiovascular risk in t Americans living with diabetes.
Interventions
DIETARY_SUPPLEMENTMitoQ (mitoquinol mesylate)
During each testing session, the investigators will be measuring how one treatment of an over-the-counter antioxidant supplement affects blood flow and muscle function. One day participants will orally ingest an over-the-counter antioxidant supplement called MitoQ.
DIETARY_SUPPLEMENTPlacebo
During each testing session, the investigators will be measuring how one treatment of an over-the-counter antioxidant supplement affects blood flow and muscle function. One day participants will orally ingest an over-the-counter antioxidant supplement called MitoQ, while the other they will ingest a placebo
Sponsors
Medical College of Wisconsin
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
TRIPLE (Subject, Investigator, Outcomes Assessor)
Intervention model description
Participants will be randomized to either active or placebo supplement, acting as their own control
Eligibility
Sex/Gender
ALL
Age
18 Years to 80 Years
Healthy volunteers
Yes
Inclusion criteria
* Type 2 Diabetes: 18 - 80 years old, able to give informed consent, \> 6 months post-diagnosis, \> 6 months stable medications for management * Healthy controls: 18 - 80 years old, able to give informed consent
Exclusion criteria
* Type 2 Diabetes: Type 1 diabetes, symptomatic coronary artery disease, cardiovascular event in last year (MI, stroke), uncontrolled or unmanaged hypertension (\>160/90 mmHg), heart failure, renal impairments, current or recent (\<6 months) tobacco use, hormone replacement therapy, documented neuromuscular disorders, pregnancy * Healthy Controls: Same as listed in Type 2 Diabetes with Type 2 Diabetes as an exclusion factor
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Mean Arterial Pressure | Prior to and 45 minutes following supplement | Change in mean arterial pressure is measured in response to handgrip exercise and post-exercise circulatory occlusion |
| Heart Rate | Prior to and 45 minutes following supplement | Change in heart rate is measured in response to handgrip exercise and post-exercise circulatory occlusion |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Brachial Artery FMD | Prior to and 45 minutes following supplementation | Brachial artery FMD a measure of endothelial function will be measured on the non-dominant arm |
| Plasma MitoQ Concentration | Prior to and 45 minutes following supplementation | Circulating concentrations of MitoQ is assessed in blood via venipuncture |
| Muscle Tissue Oxygenation | Prior to and 45 minutes following supplementation | We will also place a near-infrared spectroscopy (NIRS) pad on the thenar eminence of the non-paretic hand to non-invasively measure tissue oxygenation via infrared light following the pneumatic forearm cuff inflation/deflation. |
Countries
United States
Outcome results
None listed