Prevalence of Hemoglobinopathies Associated With Significant Hb Variants in the Chattogram Region of Bangladesh

Prevalence of Hb Variants and Haemoglobinopathies in a Tertiary Care Hospital: A Retrospective Cross-sectional Study

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT07207551

Acronym

Hb variants

Enrollment

760

Registered

2025-10-06

Start date

2023-06-01

Completion date

2025-09-04

Last updated

2025-10-06

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Hemoglobinopathies

Keywords

Hb Variants, HbE Trait, Beta Thalassemia, Iron Profile, Peripheral Blood Film (PBF)

Brief summary

Hemoglobinopathies represent a collection of genetic conditions that influence the structure or synthesis of haemoglobin, the protein found in red blood cells that facilitates oxygen transport from the lungs throughout the body. This research proposal presents a study evaluating the prevalence of hemoglobinopathies and their significant haemoglobin variants within a specific population. The research will employ a cross-sectional study design, recruiting participants through community outreach efforts and healthcare facilities while obtaining informed consent. Data will be collected with the utmost precision and rigor, using advanced screening techniques such as high-performance liquid chromatography (HPLC), capillary electrophoresis, and molecular analysis. The gathered data will be processed to assess haematological parameters, including RBC, Iron Ferritin, TIBC, electrophoresis profiles (HbA, HbA2, HbE, HbF), MCV, MCHC, and PCV among individuals with hemoglobinopathies (HbE disease/trait, Beta thalassemia disease/trait, heterozygous HPFH, HbE-beta thal). They will examine variations relative to demographic factors. The expected results will have substantial clinical and public health consequences by enhancing genetic counselling, informing clinical decision-making, and strengthening public health initiatives.

Detailed description

Hemoglobinopathies are genetic disorders affecting the structure or production of hemoglobin, the oxygen-carrying protein in red blood cells. This study aims to determine the prevalence and major variants of hemoglobinopathies in a target population using a cross-sectional design. Participants will be recruited through community outreach and healthcare facilities with informed consent. Screening will involve advanced techniques such as HPLC, capillary electrophoresis, and molecular analysis, alongside hematological assessments (RBC, ferritin, TIBC, MCV, MCHC, PCV, and hemoglobin profiles: HbA, HbA2, HbE, HbF). Findings will be analyzed in relation to demographic factors.

Interventions

None listed

Study design

Observational model

CASE_ONLY

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Healthy volunteers

Inclusion criteria

* Residents of the Chattogram metropolitan area. * All ages and both sexes with informed consent. * Willing to provide venous blood samples. * No blood transfusion in the past 3 months.

Exclusion criteria

* Refusal or withdrawal of consent. * Blood transfusion within the last 3 months. * Pregnant women. * Inadequate or hemolyzed blood samples.

Design outcomes

Primary

Measure	Time frame	Description
Detection of Hemoglobin Variants by Hb-Electrophoresis	Day 1, at enrollment	Performed to detect hemoglobin variants (e.g., HbA, HbE, HbS, HbC, HbD, HbF). The outcome will report the number and percentage of participants with each variant.

Secondary

Measure	Time frame	Description
Correlation of Hematological Parameters with Demographic Factors	Day 1, upon enrollment	Statistical correlation between hematological indices and demographic factors (eg. age, sex, family history).

Countries

Bangladesh

Outcome results