Healthy Adults; Hematopoietic System; Caffeine
Conditions
Keywords
Caffeine consumption, caffeine, Hematopoietic Stem Cells, Hematopoietic Stem and Progenitor Cells, Effect of caffeine on blood system, Effect of caffeine on hematopoietic system
Brief summary
This study investigates how caffeine intake affects blood stem and progenitor cells in healthy adults. The trial will compare people who regularly consume caffeine with those who consume very little or none. All participants will receive a single 200 mg caffeine tablet (similar to one cup of coffee) under fasting conditions. Blood samples will be collected before and three hours after caffeine intake. The study will assess whether caffeine influences the mobilization of blood stem and progenitor cells from the bone marrow into the bloodstream. It will also examine the effects of caffeine on the function, gene activity, and metabolism of blood cells.
Interventions
OTHERCaffeine (200 mg)
Participants receive a single oral dose of 200 mg caffeine under fasting conditions. Blood samples are collected before and three hours after intake to evaluate effects on hematopoietic stem and progenitor cells, including mobilization, function, gene expression, and metabolism.
Sponsors
ETH Zurich
Laboratory of Stem Cell Biology and Ageing, ETH Zurich, Prof. Nina Cabezas-Wallscheid
Prof. Dr. Nina Cabezas-Wallscheid, ETH Zurich
Jasmin Rettkowski
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
This is a single-group, open-label study in which all participants receive a single oral dose of 200 mg caffeine under fasting conditions. Blood samples are collected before and three hours after caffeine intake to assess mobilization, function, gene expression, and metabolic profile of hematopoietic stem and progenitor cells.
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Able to give consent. * Healthy adults aged ≥ 18 years. * Regular caffeine consumers (≥1 coffee cup/day or ≥1 energy drink/day) or non-consumers (≤1 cup/month and ≤1 energy drink/month) for at least 6 months. * Willingness to comply with fasting requirements and study procedures.
Exclusion criteria
* Chronic illnesses or medication use affecting metabolism, including metabolic disorders (e.g., diabetes, thyroid dysfunction, metabolic syndrome). * Personal history of cancer. * Personal history of hematological disorders. * History of radiation therapy. * Known caffeine hypersensitivity or intolerance. * Known immunosuppression.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Mobilization of hematopoietic stem and progenitor cells | From baseline to three hours after caffeine intake | The change in the numbers of mobilized HSPCs (defined as CD34+ cells) \[cells/ml\] in blood (measured by flow cytometry) from baseline to three hours after intake of a single oral dose of 200mg caffeine in habitual caffeine consumers versus non-caffeine consumers. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Functional alterations in CD34+ peripheral blood cells | From baseline to three hours after caffeine intake | Functional alterations in CD34+ PB cells: self-renewal \[number of colonies\] assessed via colony-forming unit assay. |
| Transcriptional changes in CD34+ cells | From baseline to three hours after caffeine intake | Transcriptional changes in CD34+ cells \[log2 fold change in gene expression and DEG count\] assessed via single-cell RNA sequencing. |
| Relative metabolite abundance | From baseline to three hours after caffeine intake | Relative metabolite abundance \[arbitrary units based on peak area or height in LC-MS\] in plasma and intracellularly in isolated CD34+ cells. |
| Change in the numbers of peripheral blood cells | From baseline to three hours after caffeine intake | The change in the numbers of PB cells \[cells/ml\], including B cells, T cells, myeloid cells in blood, and relative, and measured by flow cytometry |
Countries
Switzerland
Outcome results
None listed