BDNF, Healthy Young Adult, Irisin, Lactate, Resistance Exercise, Stroop Test
Conditions
Brief summary
The aim of this study is to investigate different resistance training loads on BNDF and irisin levels in platelets, blood serum and plasma.
Detailed description
In this study, the investigators will examine how three resistance training protocols acutely affect BDNF levels (in serum, plasma, platelet count, and BDNF per platelet) and irisin levels in blood (plasma). The resistance training protocols will differ in intensity, rest intervals between sets and repetition execution. Blood samples will be collected from participants at four time points: before the start of the protocol, 5 minutes after completion of the final set, 1 hour after completion of the final set, and 3 hours after completion of the final set. In addition, blood lactate levels will be monitored at the same time points. Heart rate will also be recorded (immediately before the start of the protocol and 30 seconds after completion of the final set). A control protocol will also be implemented, in which blood will be collected at the same time points. Data will be collected across five visits to the kinesiology laboratory at the University of Ljubljana, Faculty of Sport. Each visit will last approximately 4 hours, except for the first visit where 1RM will be measured.
Interventions
OTHERExercise: 80% 1 RM with 2 minutes break
Participant will do resistance exercise at 80% 1RM with 2 minutes break, 4 sets of each exercise. Included exercises are lat pull, bench press and leg press.
OTHERExercise: 80% 1RM with1 minute break
Participant will do resistance exercise at 80% 1RM with 1 minutes break, 4 sets of each exercise. Included exercises are lat pull, bench press and leg press.
OTHERExercise: accenuated eccentric exercise
Participant will do accenuated eccentric resistance exercise at 80% 1RM in concetric part of movement and 110% 1RM in eccentric part of movement with 2 minutes break, 4 sets of each exercise. Included exercises are lat pull, bench press and leg press.
OTHERControl
Timeline of events will be same as in exercise protocol (blood withdrawal and Stroop test), except participants will not do any kind of exercise protocol, instead they will be seating.
Sponsors
University of Ljubljana
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
18 Years to 30 Years
Healthy volunteers
Yes
Inclusion criteria
* males between 18 and 30 years * moderate level of physical activity assessed with IPAQ
Exclusion criteria
* injuries * cardiovascular diseases * respiratory diseases * psychiatric diseases * metabolic diseases * other neurodegenerative diseases
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Irisin | pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise. | Irisin will be measured in blood plasma. |
| Platelet count | pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise. | Platelet count in blood will be done. |
| BDNF per platelet | pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise. | The amount of BDNF per platelet will be calculated. |
| plasma BDNF | pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise. | BDNF will be determined in blood plasma with ELISA kit. |
| serum BDNF | pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise. | BDNF will be determined in blood serum with ELISA kit. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Stroop test | pre, 15 minutes post exercise. | Stroop test will be used as an assessment of cognitive functions. The number of read word will be assessed. |
| Lactate | pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise. | Lactate will be measured in blood. |
Countries
Slovenia
Outcome results
None listed