No Condition, Assessment of Healthy Volunteers
Conditions
Keywords
Cardiorespiratory fitness, acute exercise
Brief summary
This study aims to examine the effects of acute aerobic exercise and cardiorespiratory fitness on cellular metabolism of CD8+ T cells and regulatory T cells (Tregs) found in the peripheral blood of humans. In addition, the study will investigate whether the effects of exercise differ based on exercise intensity, as well as whether the effects of exercise and fitness differ between subpopulations of CD8+ T cells and Tregs. Finally, the study aims to examine whether exercise- and fitness-induced changes in cell metabolism relate to changes in cell function.
Interventions
BEHAVIORALAcute exercise-moderate intensity
30 minutes of cycling exercise at moderate (90% of first ventilatory threshold)
BEHAVIORALAcute effects-heavy intensity
Participants will cycle 30 minutes at heavy (110% of first ventilatory threshold) intensity
Sponsors
University of Houston
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
20 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Men and women, aged 20-40 years * individuals presenting with average (40th-60th percentile) cardiorespiratory fitness (i.e., VO2max/VO2peak) for their age and sex will be included in acute exercise experiments; individuals with VO2max/VO2peak value \>20ml/kg/min will be included in cardiorespiratory fitness experiments * individuals categorized as 'low risk' for a cardiovascular event/heart disease when screened by American College of Sports Medicine (ACSM) criteria
Exclusion criteria
* adults under the age of 20 and over the age of 40 * Individuals with contraindications to exercise testing will be excluded * Individuals with musculoskeletal impairments preventing them from exercising on a cycle ergometer * Individuals with disease history known to impact the immune system and/or systemic metabolism will be excluded, including autoimmune diseases, HIV, Hepatitis, diabetes, cancer (completed treatment within \<6 months prior), hyper- or hypothyroidism * Pregnancy * Individuals using medications known to impact the cardiopulmonary and/or immune response to exercise with be excluded, including those using medication to control blood pressure (e.g., beta-blockers), daily steroidal and nonsteroidal anti-inflammatories (except daily aspirin), prescription allergy medication, ADHD medication (Ritalin or Adderall), thyroid medication (e.g., Levothyroxine), and chemotherapy (including treatments within the last 6 months). * Individuals habitually using tobacco products within the last 6 months or with excessive alcohol intake (\>10 drinks/week)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Metabolic Function | At rest, immediately after, and two hours after exercise | Cellular metabolic activity and poise of T cells as measured by flow cytometry (SCENITH) |
| Immune Cell function | At rest, immediately after, and two hours after exercise | Stimulated IFN-gamma, perforin and IL-10 production, as measured by flow cytometry |
| Metabolic Regulation | At rest, immediately after, and two hours after exercise | Expression of genes involved in cellular energy metabolism, as assessed by RT-PCR |
| Mitochondrial Regulation | At rest, immediately after, and two hours after exercise | Mitochondrial mass and membrane potential, as assessed by flow cytometry |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Peripheral immune cell composition | At rest, immediately after, and two hours after exercise | Number (concentration) of immune cell subsets in peripheral blood, as measured by hematology analyzer and flow cytometer |
Countries
United States
Outcome results
None listed