Endometriosis
Conditions
Keywords
endometriosis, biomarkers, implantation, infertility, fertility
Brief summary
This study aims to explore biomarkers in the endometrial fluid and tissue of women with moderate to severe endometriosis, with a specific focus on those involved in pre-receptive endometrium and embryo implantation. This research has the potential to uncover diagnostic indicators of impaired pre-receptive endometrium, examine pathophysiological pathways, and identify future targets for therapeutic intervention. Ultimately, the findings may contribute to more personalized fertility treatments, offering new hope for women struggling with endometriosis-related infertility.
Interventions
DIAGNOSTIC_TESTEndometrial fluid
Endometrial fluid collection in the day of OPU
DIAGNOSTIC_TESTEndometrial biopsy
Endometrial biopsy on the OPU day, Pipelle
DIAGNOSTIC_TESTFollicular fluid
Follicular fluid sampling on the OPU day
Sponsors
Region Stockholm
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Age 18-40 years old * Diagnosed with moderate/severe endometriosis by ultrasound or laparoscopy (patient group) * Regular menstrual cycles 28-35 days * Speak English or Swedish and agree to participate in the study
Exclusion criteria
* Pregnancy or lactation in the last 3 months * Uterine anomalies * Pelvic inflammatory disease, endometritis, STD * Gynaecological cancers or other malignancies in the patient's history * Smoking (more than 10 cigarettes per day) or alcohol abuse * Immunosuppressing medicines * Major abdominal or pelvic surgery in the last 3 months * A specific genetic disease that is diagnosed before PGT in women undergoing treatment
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Relative expression levels (e.g., fold change, normalized intensity, or concentration in pg/mL/ng/mL depending on biomarker assay) | 2 years | Evaluation of the expression levels of selected biomarkers in endometrial fluid samples from patients with moderate to severe endometriosis compared to healthy controls. |
| Percentage of blastoids successfully implanted in vitro (% implantation rate) | 2 years | Evaluation of the implantation efficiency of blastoids in vitro (endometrial organoids) under controlled laboratory conditions. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Relative expression levels (e.g., concentration in pg/mL, ng/mL, or fold change depending on assay) | 2 years | Assessment of biomarker expression levels in follicular fluid samples from follicles in different distances from endometriomas in patients with severe endometriosis. |
Countries
Sweden
Contacts
Primary ContactAnastasia Drakou, MSc, PhD student
Backup ContactKenny Rodriguez-Wallberg, Professor
Outcome results
None listed