Polycystic Ovary Syndrome (PCOS)
Conditions
Keywords
PCOS, Steroid Profiling, Oral Contraceptives, LC-MS/MS, Adolescents, Hyperandrogenism
Brief summary
This prospective observational study investigates the changes in serum steroid intermediates assessed by liquid chromatography-mass spectrometry (LC-MS/MS) in adolescents and young women with polycystic ovary syndrome (PCOS), before and after oral contraceptive (OCP) treatment.
Detailed description
The study enrolled 56 female patients aged 13-21 years with a diagnosis of PCOS according to Rotterdam criteria. At baseline, all participants underwent LC-MS/MS steroid profiling. A subgroup of 15 patients received oral contraceptive therapy based on clinical indication, independent of the study protocol. A second LC-MS/MS steroid analysis was performed at 6 months in this subgroup. The primary aim was to investigate the effect of OCP on the steroid biosynthesis profile. The study was approved by the Istanbul University Ethics Committee (Approval ID: 2019/822) and conducted between January 2020 and December 2021.
Interventions
None listed
Sponsors
Istanbul University
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
13 Years to 21 Years
Healthy volunteers
No
Inclusion criteria
* Diagnosis of PCOS based on Rotterdam criteria * Age between 13 and 21 years * No hormonal treatment in the 3 months prior to study enrollment * Consent provided
Exclusion criteria
* Congenital adrenal hyperplasia * Cushing's syndrome * Pregnancy * Use of hormonal medications within 1 year prior to baseline
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Serum adrogen levels | 6 months | Change in serum androgen levels (e.g., testosterone, androstenedione, DHEAS) measured by LC-MS/MS between baseline and 6 months in the OCP-treated group. |
| serum androgen levels | 6 months | Change in serum androgen levels (e.g., testosterone, androstenedione, DHEAS) measured by LC-MS/MS between baseline and 6 months in the OCP-treated group. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Change in serum steroid hormone levels after 6 months of OCP treatment in adolescents with PCOS | Baseline and 6 months after the initiation of OCP treatment | Comparison of steroid hormone profiles measured by LC-MS/MS between the baseline and 6-month follow-up in adolescent PCOS patients who were treated with oral contraceptive pills (OCP). This outcome aims to assess the impact of OCP therapy on specific androgenic and progestogenic steroid metabolites. |
Countries
Turkey (Türkiye)
Outcome results
None listed