Sensitive Scalp
Conditions
Keywords
sensitive scalp
Brief summary
Sensitive skin is defined as a syndrome manifested by the occurrence of unpleasant sensations (tingling, burning, pain, pins and needles) in response to stimuli that should not normally cause them. These unpleasant sensations cannot be explained by lesions attributable to a specific skin disease. Sensitive skin can affect different parts of the body. The scalp is a site that is often affected, with specificity linked in particular to the presence of hair and different triggering factors (styling habits, wearing of head coverings, application of cosmetics to the scalp, etc.). Sensitive scalp affects around half the population, and can have an impact on the quality of life of sufferers, particularly those whose symptoms are very intense. Women are more likely than men to have a sensitive scalp, so in order to have a more homogenous study population, we chose to include 40 women. The pathophysiology of sensitive skin is imperfectly understood, and studies specific to the sensitive scalp are very rare. However, the pathophysiology of the sensitive scalp could be different because it is a hairy area, more innervated, and less exposed to environmental factors.
Detailed description
Each patiente will have a sample taken in the dermatology department: * 1 skin biopsy of the scalp (non-routine medical procedure) * Completion of questionnaires * Collection of skin microbiota (optional) 40 adult women * 20 women with a sensitive scalp (cases) * 20 women without a sensitive scalp (controls) Patientes with the sensitive scalp will be recruited from the dermatology department. There will be no further visits, so the duration of the study is 1 day.
Interventions
PROCEDUREA scalp biopsy using a 4 mm punch in the retroauricular hair zone
a scalp biopsy using a 4 mm punch in the retroauricular hair zone under local anesthesia
OTHERa general health questionnaire
a general health questionnaire : demographics, history, treatment, smoking, alcohol consumption, phototype.
OTHERQuestionnaires concerning their sensitive scalp
* The characteristics of a sensitive scalp (location, chronicity, triggering and soothing factors, use of cosmetics, etc.) * Sensitive Scalp Score (3S) * Impact on quality of life: BoSS questionnaire * Impact of pruritus on quality of life (ItchyQol)
PROCEDURECollection of skin microbiota
The skin microbiome is collected using a sterile swab rubbed against the scalp for 60 seconds
Sponsors
University Hospital, Brest
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
PREVENTION
Masking
NONE
Masking description
Histological and innervation analyses will be carried out in a blinded fashion.
Intervention model description
case-control study
Eligibility
Sex/Gender
FEMALE
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Adult women without dermatosis * Collection of free and informed consent * patient affiliated to a social security scheme For the control group: Sensiscalp score = 0/20 Sensitive scalp group: Sensiscalp score ≥ 3/20 with pruritus sensation ≥ 2/20
Exclusion criteria
* Refusal to take part in the study * Dermatosis of the scalp (psoriasis, seborrhoeic dermatitis, etc.) * Pregnant and breast-feeding women * Women under legal protection (guardianship, curatorship)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Study the pathophysiology of the sensitive scalp based on a study of the genes expressed in the cutaneous tissue | 1 day | 1. mRNA in biopsy by bulk transcriptomic -\> Identify differentially expressed genes between the two groups (sensitive scalp and non-sensitive scalp) 2. mRNA in biopsy by RNAscope -\> Quantify and localized expression of interest's genes |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Histological study of skin tissue | 1 day | 1. Histological features in biopsies - Routine staining -\> Visualize/Compare skin architecture between the 2 groups (sensitive scalp and non-sensitive scalp), 2. Protein expression in biopsies - immunofluorescent staining -\> Compare the status of keratinocytes between the two groups (sensitive scalp and non-sensitive scalp) 3. Protein expression in biopsies - immunofluorescent staining -\> Compare the Receptors and ion channels expression known to be involved in pruritus between the 2 groups (sensitive scalp and non-sensitive scalp) |
| Study of innervation | 1 day | 1\) Protein expression in biopsies - immunofluorescent staining -\> Quantify free nerve endings in the epidermis and the dermis and compare between the 2 groups (sensitive scalp and non-sensitive scalp) |
| Study of the skin microbiome | 1 day | Quantification and characterization of microorganisms |
Countries
France
Contacts
CONTACTEmilie BRENAUT, Dr
Outcome results
None listed