Protein Metabolism
Conditions
Keywords
Protein quality, protein metabolism, stable isotopes, protein oxidation, indicator amino acid oxidation, protein synthesis
Brief summary
Given the relatively high carbon footprint and sustainability of animal-based proteins, there is a growing interest in determining the nutritional quality non-animal-based protein sources. The overall objective of this investigation is to examine the impact of different animal and microbial-based protein sources to support whole body protein synthesis in adults. To do this, investigators will employ a 'breath test' method developed in our laboratory as well as urine sampling. The results of this study will allow us to better understand the impact of dietary protein quality for maintaining health and body protein mass in adults.
Interventions
DIETARY_SUPPLEMENTYeast Protein Supplement
Each protein will be consumed at 0.9g/kg/d in n=8 hourly drinks
DIETARY_SUPPLEMENTWhey Protein Isolate Supplement
Each protein will be consumed at 0.9g/kg/d in n=8 hourly drinks
DIETARY_SUPPLEMENTCollagen Hydrolysate Supplement
Each protein will be consumed at 0.9g/kg/d in n=8 hourly drinks
Sponsors
Lesaffre International
Daniel Moore
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Subject)
Intervention model description
Single-blind randomized, counterbalanced design Thirteen healthy young (18-45 years of age, at least 6 females and 6 males) will be subjected to a non-invasive 13CO2 breath-test based on the indicator amino acid oxidation method and urine collections following hourly ingestion of protein test beverages (whey, yeast, and collagen randomized by trials).
Eligibility
Sex/Gender
ALL
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy, male and female, recreationally-active participants. * Healthy will be defined as screened by the PAR-Q+ (Appendix B) and The Physical Activity Readiness Questionnaire (Appendix C). * Participants will be aged 18-45 years old. * Participants are willing to abide by the compliance rules of this study. * Self-reported regular menstrual cycle (25-35d) within the last 3 months (female participants). * Use of monophasic combined oral contraceptives (COC) containing 21 active pills (e.g., Yaz, Marvelon, Cyclen, etc.; the brand of COC will be recorded) (female participants).
Exclusion criteria
* Inability to adhere to any of the compliance rules judged by principal investigator (e.g. dairy protein or yeast allergy). * Self-reported regular tobacco use. * Self-reported illicit drug use (e.g., growth hormone, testosterone, etc.). * Individuals who have participated in studies within the past year involving any of the stable isotopes in the study. * Use of multiphasic COCs due to the difficulty of controlling for hormonal fluctuations in such formulations (female participants). * Use of COCs containing \>21 active pills per cycle due to potential differences in their effect on metabolism (female participants). * Use of other forms of hormonal contraception (e.g., progestin-only pill, hormonal IUD, intravaginal ring) (female participants). * Not currently or previously (in the past 6 months) on a vegan diet
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| 1. Phenylalanine excretion (umol/kg/h) | 8 hours | Whole body phenylalanine excretion determined from the product of breath 13CO2 enrichment and carbon dioxide production rate (VCO2). |
| Phenylalanine oxidation (umol/kg/h) | 8 hours | Whole-body phenylalanine oxidation determined from the correction of phenylalanine excretion by the phenylalanine precursor enrichment determined from urine enrichment over the 8 hour measurement period. |
Countries
Canada
Contacts
Primary ContactDaniel R. Moore, Ph.D.
Outcome results
None listed