Oxidative DNA Damage Enhancement by Vitamin C Among Narghile Smokers and Non-smokers

Status

Completed

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT07141823

Acronym

DNA Damage

Enrollment

Registered

2025-08-26

Start date

2024-10-15

Completion date

2025-03-10

Last updated

2025-08-26

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Narghile

Keywords

DNA damage, Oxidative stress ,MDA, vitamin C

Brief summary

This study aims to evaluate oxidative DNA damage among hookah smokers compared to non-smokers by measuring biomarkers of oxidative stress in blood samples. The objective is to determine the impact of hookah smoking on DNA integrity and overall oxidative status in adults.

Detailed description

Hookah smoking has become increasingly popular in many regions, yet its potential impact on oxidative stress and DNA integrity is not fully understood. This study aims to investigate the level of oxidative DNA damage among hookah smokers compared to non-smokers by analyzing biomarkers in blood samples. A case-control observational design will be used, involving two groups of adult participants: regular hookah smokers and age-matched non-smokers. Blood samples will be collected from all participants and analyzed for markers of oxidative stress, including 8-hydroxy-2'-deoxyguanosine (8-OHdG) and malondialdehyde (MDA), using validated biochemical methods. The findings are expected to provide insights into the biological consequences of hookah smoking and support the development of public health interventions.

Interventions

DIETARY_SUPPLEMENTVitamin C (Ascorbic Acid) regularly has been taken

Study design

Allocation

RANDOMIZED

Intervention model

PARALLEL

Primary purpose

BASIC_SCIENCE

Masking

SINGLE (Subject)

Eligibility

Sex/Gender

ALL

Age

18 Months to 30 Months

Healthy volunteers

Inclusion criteria

* Adults aged 18-30 years Current narghile (hookah) smokers for at least 1 year Willing to provide informed consent

Exclusion criteria

* Healthy participant

Design outcomes

Primary

Measure	Time frame	Description
Oxidative DNA damage	Baseline and after 1 month	Measurement of plasma MDA concentration using the thiobarbituric acid reactive substances (TBARS) method to assess oxidative stress in participants before and after 1-month Vitamin C supplementation and measuring oxidative stress marker and TAOC

Countries

Iraq

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026