Oxidative Stress, Sepsis
Conditions
Keywords
Diagnostic efficacy, Sepsis, Oxidative Stress, Emergency Department, Thiol- Disulfide Homeostasis
Brief summary
The aim of this study was to determine the effectiveness of thiol-disulfide homeostasis in predicting diagnosis and mortality in sepsis patients, and to compare it with other inflammatory markers.
Detailed description
Sepsis is a serious illness with high mortality rates triggered by infection-associated pathogenesis involving oxidative stress dysfunction. This study aims to elucidate the diagnostic efficacy of changes in thiol-disulfide homeostasis, its predictive role in mortality, relationship with other inflammatory parameters in sepsis patients in emergency department. In prospective, randomized controlled study, 50 patients diagnosed with sepsis according to the Sepsis-3 guidelines who presented emergency department were compared with a control group of 50 healthy volunteers. Thiol-disulfide homeostasis parameters, including total thiol, native thiol, disulfide levels, reduced thiol, oxidized thiol, and thiol oxidation-reduction ratios, were examined. Additionally, inflammatory blood parameters (C-reactive protein, procalcitonin, lactate) and mortality durations were compared with thiol-disulfide homeostasis. The objective of this study was to examine the effectiveness of thiol-disulfide homeostasis in forecasting diagnosis and mortality in sepsis patients, and to compare it against other inflammatory markers.
Interventions
DIAGNOSTIC_TESTThiol- Disulfide Blood Sample Measurement
Measurement of thiol-disulfide homeostasis parameters and inflammatory markers from serum samples.
Sponsors
Sakarya University
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
DIAGNOSTIC
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Patients aged 18 and above who met the diagnostic criteria were included in the study
Exclusion criteria
* Who did not meet the criteria for sepsis diagnosis according to the quick Sequential Organ Failure Assessment score * Individuals with malignancy, acute coronary syndrome, acute cerebrovascular event, acute/chronic kidney/liver failure, rheumatologic diseases, febrile neutropenia, immunosuppressive disorders, corticosteroid use, and those * Individuals with a history of antibiotic use within the last week
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Diagnostic value of serum thiol-disulfide homeostasis parameters in sepsis | 2 monhts | Measurement of serum total thiol, native thiol, disulfide, reduced thiol, and oxidized thiol levels in patients with sepsis and healthy controls at the time of admission, and comparison between groups to evaluate diagnostic performance. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| General Characteristics of All Participants | 1 week | Demographic data (age, gender, body mass index) were recorded. |
| Correlation of inflammatory and biochemical markers with thiol-disulfide homeostasis in sepsis | 10 days | Initial blood samples collected from sepsis patients were analyzed to measure total thiol, native thiol, disulfide, reduced thiol, and oxidized thiol levels. These thiol-disulfide homeostasis parameters were then compared and correlated with standard laboratory inflammatory markers, including C-reactive protein (CRP), procalcitonin, and lactate, to evaluate their relationships. |
Countries
Turkey (Türkiye)
Outcome results
None listed