Infertility Assisted Reproductive Technology
Conditions
Keywords
interleukin, ngs, art, icsi, implantation, euploid, uterine immunity
Brief summary
Evaluation of uterine immunity, histological picture, and endometrial microbiome for embryo implantation
Interventions
None listed
Sponsors
University of Bari
University of Florence
Casa Sollievo della Sofferenza-IRCCS, San Giovanni Rotondo
Momo Fertilife
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
20 Years to 42 Years
Healthy volunteers
Yes
Inclusion criteria
Female patients aged \>18 and \<43 years at the time of embryo transfer. Transfer of cryopreserved embryos diagnosed as euploid through preimplantation genetic testing for aneuploidy (PGT-A). Embryo transfer performed at the blastocyst stage.
Exclusion criteria
Patients with an intrauterine device (IUD) in situ within the three months prior to study enrollment. Presence of autoimmune diseases and/or ongoing treatment with corticosteroids. Structural or pathological abnormalities of the uterine cavity, including but not limited to polyps, intramural myomas measuring 2-4 cm, submucosal fibroids, uterine septum, or hydrosalpinx, identified during study participation. Patients with a history of these conditions may be included if the pathology has been resolved prior to the initiation of any study-related procedures or sample collection. Presence of severe or uncontrolled fungal or viral infections which, in the opinion of the principal investigator, may compromise the patient's ability to participate or affect the integrity of study outcomes. Any medical condition or illness that is unstable, poses a potential risk to patient safety, or may interfere with adherence to the study protocol. Patients lacking a confirmed preimplantation genetic diagnosis of euploidy. Patients with a clinical diagnosis of endometriosis or adenomyosis.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Characterise the molecular immunologic mechanism underlying embryo implantation | from enrollment to the pregnancy test (12 days after embryo transfer) | The main objective of this research is to characterise the molecular immunologic mechanism underlying embryo implantation. These outcomes will be assessed regarding the percentage of expressivity of some interleukins in pregnant couples and those who are not |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| to study the possible correlations of the expression of the interleukins involved in the implantation process of the embryo | The endometrial biopsy on which the interleukin dosage will be done, will be taken one month before the embryo transfer | to study the possible correlations of the expression of the interleukins involved with related anatomical and histological pictures. Interleukin dosage will be assessed through PCR. The unit of measure is ng/ml. |
Other
| Measure | Time frame | Description |
|---|---|---|
| to assess whether progesterone levels can alter the immune response | the same day of the embryo transfer | immunofluorescence device; unit of measure: ng/ml |
Countries
Italy
Outcome results
None listed