Healthy Subjects or Volunteers
Conditions
Keywords
Hydrogen-rich water, Resting metabolism, Energy expenditure, Substrate utilization, Metabolic rate
Brief summary
The H2REST trial is a randomized, double-blind, placebo-controlled crossover study investigating the acute effects of a single dose of hydrogen-rich water (HRW) on resting metabolism in healthy young adults. Participants complete two testing sessions-receiving either HRW or placebo in a randomized order-followed by standardized metabolic testing. Primary outcomes include resting energy expenditure, respiratory exchange ratio, and substrate utilization measured via indirect calorimetry. The study aims to assess whether acute HRW intake can modulate metabolic rate and fuel preference at rest, and to explore inter-individual variability in response.
Interventions
DIETARY_SUPPLEMENTMolecular Hydrogen
Participants in this arm will consume a single 250 mL dose of hydrogen-rich water containing approximately 1.6-2.0 ppm of dissolved molecular hydrogen (H₂), The HRW will be ingested following an overnight fast, and resting metabolism will be measured approximately 30-60 minutes post-consumption using indirect calorimetry.
DIETARY_SUPPLEMENTPlacebo Comparator
Non-hydrogen-producing tablets, identical in appearance and taste to the active supplement
Sponsors
University of Novi Sad
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
TRIPLE (Subject, Caregiver, Investigator)
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Aged 18-35 years * BMI between 18.5 and 25.0 kg/m² * Apparently healthy, with no history of chronic disease * Non-smoker, not currently using nicotine-containing products * Able to provide informed consent * Willing to refrain from alcohol, caffeine, and strenuous exercise for 24 hours before each study visit * Able to comply with study procedures, including fasting and attending all study visits
Exclusion criteria
* Diagnosed metabolic, cardiovascular, respiratory, gastrointestinal, or endocrine disorders * Use of dietary supplements or medications affecting metabolism (within the past 2 weeks) * Allergy or intolerance to components of the hydrogen or placebo beverages * Pregnant or lactating * Shift work or irregular sleep schedule * Participation in another clinical study within the past 30 days
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Resting Energy Expenditure (REE) | Participants will attend three study visits, with each intervention separated by a 7-day washout period to minimize carry-over effects. During each visit, measurements will be conducted approximately 30-60 minutes post-intervention. | The measurements will be obtained during each study visit. Since this is an interventional study assessing the acute effects of molecular hydrogen, measurements will be collected three times: At baseline, without any intervention (pre-familiarization), After a single dose of molecular hydrogen, and After placebo administration. The period between each intervention will be seven days, serving as a washout period to prevent carry-over effects. All participants will be instructed to maintain their regular diet and to avoid alcohol, caffeine-containing beverages, and strenuous physical activity for at least 24 hours prior to each visit. Measure Type: Continuous (kcal/day) Method: Indirect calorimetry Description: To assess the effect of acute ingestion of hydrogen-rich water versus placebo on resting energy expenditure in healthy young adults. |
Contacts
Primary ContactNikola Todorovic, PhD
Outcome results
None listed