Brain Injury, Neutrophils/Lymphocytes Ratio, Prediction, S100B
Conditions
Brief summary
The study aimed to evaluate the role of Protein S100B versus the neutrophil-to-lymphocyte ratio (NLR) as an early predictor biomarker and the value of amplitude-integrated electroencephalography (aEEG) as an early predictor and prognostic method for neonatal brain injury susceptibility and severity.
Detailed description
The most prevalent types of newborn brain injury (NBI) are intraventricular haemorrhage (IVH), periventricular leukomalacia (PVL), and hypoxic-ischemic encephalopathy (HIE), which can affect neonates born at any gestational age (GA). Amplitude-integrated EEG (aEEG) is another early predictor and prognostic technique for Neonatal Brain Injury. Electrophysiological brain activity, as measured by aEEG, is a well-established method for giving information on the functional and metabolic status of the brain and the incidence of epileptic seizure episodes. S100B belongs to the S100 family. Because it is soluble in a 100% saturated solution of ammonium sulfate, it was designated S100. S100 protein is a calcium-binding cytosolic protein with a molecular weight of 21 kDa comprised of 2 monomers, which are found in a variety of cells and are primarily concentrated in the glial cells of the CNS. Due to its molecular weight, peripheral blood will detect only S100B. The neutrophil-to-lymphocyte ratio (NLR) reflects changes in neutrophil and lymphocyte levels, which is an indication of inflammation.
Interventions
DIAGNOSTIC_TESTS100B Protein
Serum level of S100B Protein was measured on the first and third days of seizure activity by ELISA.
DIAGNOSTIC_TESTNeutrophils to Lymphocytes ratio
Neutrophils to Lymphocytes ratio was measured on the first and third day of seizure activity obtained by complete blood count.
Sponsors
Tanta University
Study design
Observational model
COHORT
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
28 Weeks to 36 Weeks
Healthy volunteers
No
Inclusion criteria
* Gestational age between 28 and 36 weeks. * Prematurity.
Exclusion criteria
* Intrauterine growth restriction (IUGR). * Multiple congenital anomalies. * Chromosomal abnormalities. * Preterm less than 28 weeks. * Infant of diabetic mother.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Protein S100B | Third day of seizure activity | Serum level of S100B Protein was measured on the first and third days of seizure activity by ELISA. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Neutrophils/Lymphocytes Ratio | Third day of seizure activity | Neutrophils to Lymphocytes ratio was measured on the first and third day of seizure activity obtained by complete blood count. |
Countries
Egypt
Outcome results
None listed