Bone Marrow Stem Cells, Exercise Training, Post-menopausal Women, Skeletal Muscle Recovery
Conditions
Brief summary
The goal of this interventional study is to observe the effects of bone marrow stem cell behavior in skeletal muscle of women between 60 to 75 years old after a single moderate resistance exercise session. Plant sterol Rg1, a compound known to enhance stem cell infiltration following exercise in young men will be tested against a placebo in a double blinded crossover design. The main questions to be answered are: * Does Rg1 supplementation alter serum sex hormone levels in recovery from exercise? * Does Rg1 supplementation influence bone marrow stem cell differentiation in skeletal muscles of older women? Participants will: * Pre-test body composition, maximal leg strength, blood and muscle tissue. * One hour after taking the supplement, participants will perform 4 sets of 10 repetitions of 70% of maximal strength (1RM) leg press and have two thigh muscle biopsies (0 and 24 h after exercise). * Repeat the protocol one month later on the alternative supplement. Researchers will understand whether Rg1 improves recovery response to resistance exercise in post-menopausal women.
Interventions
DIETARY_SUPPLEMENTexercise following Rg1 consumption
The effect of Rg1 on exercised skeletal muscle in older women
DIETARY_SUPPLEMENTPlacebo
Resistance exercise performed 1 hour following placebo consumption
Sponsors
University of Taipei
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
TRIPLE (Subject, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
FEMALE
Age
60 Years to 70 Years
Healthy volunteers
Yes
Inclusion criteria
* sedentary, able to cease medications and supplements for 48 h before and 24 h after exercise session, medically sound for lower body moderate resistance exercise
Exclusion criteria
* use of insulin, sex hormone and immune-modulating medications, smoking and regular alcohol consumption
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Stro-1+ bone marrow stem cell area in skeletal muscle biopsies | from enrolment to the end of study at 8 weeks | Immunofluorescence staining will be used to semi-quantitatively analyze Stro-1+ BMSC presence in skeletal muscle of older women before, 0h and 24h after a moderate resistance exercise session. |
| Nestin+ neural stem cell area in skeletal muscle biopsies | from enrolment to the end of study at 8 weeks | Immunofluorescence staining will be used to semi-quantitatively analyze Nestin+ neural stem cell presence in skeletal muscle of older women before, 0h and 24h after a moderate resistance exercise session. |
| CD34+/CD31+ vascular stem cell area in skeletal muscle biopsies | from enrolment to the end of study at 8 weeks | Immunofluorescence staining will be used to semi-quantitatively analyzeCD34+/CD31+ vascular stem cell presence in skeletal muscle of older women before, 0h and 24h after a moderate resistance exercise session. |
| Serum sex hormone levels 24 h following resistance exercise | from enrolment to the end of study at 8 weeks | Forearm blood draw and Elisa analysis of serum for estradiol, progesterone, and testosterone of older women before and 24h after a moderate resistance exercise session. |
Countries
Taiwan
Outcome results
None listed