Colorectal Cancer (MSI-H), Microplastic Exposure
Conditions
Keywords
colorectal cancer, microplastic, Paraneoplastic, Genomics, Metabolomics
Brief summary
The objective of this study is to identify and characterize microplastics present in peritumoral and intratumoral tissues of colorectal cancer patients, and to investigate their associations with gut microbiome composition and metabolic profiles. By integrating microplastic analysis with metagenomic and metabolomic data, we aim to explore the potential impact of environmental microplastic exposure on tumor microenvironment alterations, microbial dysbiosis, and metabolic reprogramming in colorectal cancer.
Interventions
OTHERexposure to microplastic
Microplastic content in cancerous and paracancerous tissues
Sponsors
Dong Peng
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Adult patients (≥18 years old) with histologically confirmed colorectal adenocarcinoma. * Undergoing elective radical colorectal cancer resection at the study hospital. * No prior chemotherapy, radiotherapy, or targeted therapy before surgery. * Availability of both tumor tissue and adjacent normal (peritumoral) tissue samples during surgery. * Willingness to provide stool and/or blood samples for metagenomic and metabolomic analysis. * Ability to understand the study procedures and provide written informed consent.
Exclusion criteria
* Patients with a history of other malignancies within the past 5 years. * Emergency surgery due to obstruction, perforation, or bleeding. * Inadequate sample quality or volume for microplastic, metagenomic, or metabolomic analysis. * Presence of chronic inflammatory bowel disease (e.g., Crohn's disease or ulcerative colitis). * Use of antibiotics, probiotics, or prebiotics within 4 weeks prior to sample collection. * Pregnant or breastfeeding women. * Refusal or inability to provide informed consent.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| genomics | Blood specimens were obtained within one week prior to surgery. | Genomics was tested by preoperative blood specimens. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| metabolomics | Blood specimens were obtained within one week prior to surgery. | Metabolomics was tested by preoperative blood specimens. |
Countries
China
Outcome results
None listed