Colorectal Carcinoma
Conditions
Keywords
p16 Expression, Colorectal Carcinoma, Immunohistochemical study
Brief summary
Evaluation of the immunohistochemical expression of p16 in colorectal carcinoma and correlate its expression to different clinical and pathological parameters as patients' ages, sexes, tumor location, histopathological phenotype, status of nodal involvement, perineural and lymphovascular invasion.
Detailed description
Colorectal Carcinoma is very common nowadays, with increasing need for further research. p16 is tumor suppressor gene with a vital rule ; inactivation of p16 leads to tumor formation and progression. 50 Specimens will be collected from Pathology Department of Sohag University Hospital. Sufficient clinical and histopathological data will be attached for each case. The previously processed formalin-fixed, paraffin-embedded tissue blocks will be used to get Hematoxylin & Eosin stained sections to confirm the diagnosis and to detect the histopathological type, grade and stage of each tumor. Another sliced tissue sections will be immunohistochemically stained with p16 monoclonal antibodies to be evaluated with the positivity, intensity and distribution of the detected stain.
Interventions
BIOLOGICALImmunohistochemical staining
Immunohistochemical staining of paraffin embedded blocks of colorectal carcinoma tissues by p16 monoclonal antibodies to be evaluated with the positivity, intensity and distribution of the detected stain.
Sponsors
Sohag University
Study design
Observational model
CASE_ONLY
Time perspective
RETROSPECTIVE
Eligibility
Sex/Gender
ALL
Healthy volunteers
No
Inclusion criteria
1. Specimens from patients with colorectal cancer. 2. Tissue blocks with sufficient material. 3. Specimens with sufficient clinical data.
Exclusion criteria
1. Tissue blocks with insufficient, destroyed or necrotic material. 2. Specimens with insufficient clinical data.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Evaluation of p16 Expression in Colorectal Carcinoma. | 6 months | Immunohistochemical staining of Colorectal tissues by p16 monoclonal antibody |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Correlation of p16 expression to different clinical and pathological parameters | 6 months | Correlation of p16 expression to different clinical and pathological parameters as patients' ages, sexes, tumor location, histopathological phenotype, status of nodal involvement, perineural and lymphovascular invasion. |
Countries
Egypt
Contacts
Primary ContactSherry R Azmy, Demonstrator of pathology
Outcome results
None listed