NT/TT Ratio, Serum Disulfide/TT Ratio, Serum Ischemia-modified Albumin (IMA), The Serum Native Thiol (NT), Total Thiol (TT)
Conditions
Keywords
infertility, in vitro fertilization, ischemia-modified albumin, male factor, thiol-disulfide homeostasis, unexplained
Brief summary
Thiol-disulfide Homeostasis (TDH) is essential for physiological functions in the human reproductive system, including antioxidant defense, detoxification, signal transduction, enzymatic regulation, apoptosis, and cellular communication. Ischemia-Modified Albumin (IMA) serves as an indicator of oxidative stress (OS) and is linked to ischemia-reperfusion injury. This study aimed to investigate changes in OS markers, particularly TDH and IMA levels, in couples with male infertility, and to examine the relationship between these markers and in vitro fertilization (IVF) success. Understanding how TDH and IMA levels influence IVF outcomes is crucial for advancing reproductive treatments for couples having diagnosis of infertility.
Detailed description
The thiol-disulfide Homeostasis (TDH) is believed to participate in critical physiological functions, including antioxidant defense, detoxification, signal transduction, enzymatic activity regulation, apoptosis, and cellular communication within the human reproductive system. Ischemia-Modified Albumin (IMA) is considered a surrogate marker for oxidative stress (OS) and has also been linked to ischemia-reperfusion injury. The present study was designed to investigate the alteration of OS markers, including TDH and IMA levels, in couples diagnosed with male infertility, and to examine the association between these OS markers and in vitro fertilization (IVF) success. Couples in which women aged 23-45 years and undergoing IVF treatment due to male factor and unexplained infertility will be enrolled. The serum native thiol (NT), total thiol (TT) levels, disulfide levels and disulfide/TT and NT/TT ratios will be compered between two groups. The serum ischemia-modified albumin (IMA) level will also compared between male infertility and unexplained infetility group. TDH related parameters as NT and TT may be useful as a reflection of OS and as potential diagnostic biomarkers for male infertility
Interventions
None listed
Sponsors
Duzce University
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
23 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
Couples in which women aged 23-45 years and undergoing IVF treatment due to male factor and unexplained infertility were enrolled. \-
Exclusion criteria
Patients with hepatic or renal disease, systemic infections, systemic inflammatory disorders (such as diabetes mellitus, thyroid hormone disorders, rheumatologic, autoimmune, endocrine, neoplastic or other inflammatory conditions), or a diagnosis of endometriosis were excluded from the study. Smokers were excluded. Additionally, women with diminished ovarian reserve (DOR) were also not included in this study. \-
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Live birth rate (LBR) | 6 months | live births to the total number of embryo transfer cycles |
| beta-human chorionic gonadotropin (hCG) positivity | 6 months | serum ≥ 10 mlU/mL |
| clinical pregnancy (CP) | 6 months | the detection of a gestational sac via transvaginal ultrasonography |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| diagnosing male infertility | 6 months | The serum native thiol (NT) levels, serum total thiol (TT) levels in males |
Countries
Turkey (Türkiye)
Outcome results
None listed