Salivary Redox Biomarkers and Oral Health
Conditions
Brief summary
The goal of this study is to test the effect of ionizing radiation on salivary Redox status among a group of ionizing radiation workers by measuring salivary biomarkers(malondialdehyde,8\_hydroxy\_deoxyguanosine and catalase) and measuring oral health condition by plaque index. 90 male subjects will be enrolled in this study between 30\_40 years old worked in Baghdad teaching hospital ,30 subjects exposed from computed tomography department ,30 subjects exposed from x\_ray department and 30 subjects unexposed from laboratory department in the same hospitals. The method of the study is taking unstimulated saliva sample from the subjects and oral health measure.
Interventions
RADIATIONSalivary redox biomarkers
Salivary redox biomarkers
Sponsors
Al-Mustansiriyah University
Study design
Observational model
CASE_CROSSOVER
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
MALE
Age
30 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* male, 30\_40 years ,no history of systemic disease, no medical treatment taken at least 3 months before sampling and the subjects are exposed to radiation 5\_ 10 years
Exclusion criteria
* subjects with medical history , subjects are taken medications during last 3 months , the subjects are exposed to radiation less than 5 years or more than 10 years
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| oral health status | Baseline: the subjects must be fasting. up to two hours saliva collection | Changes in the clinical periodontal parameter (Plaque index ) To determine the patients' plaque index, their dental plaque thickness was evaluated by probing the mesial, distal, buccal, and palatal surfaces of all teeth using a Williams periodontal probe. The individual's plaque index was determined by summing the values obtained for each tooth and calculating the averages.Silness & Löe (Silness & Löe,1964) reference values were taken as a basis The Plaque Index (PI) scores each site on a 0 to 3 scale Score 0 : No plaque is in the area adjacent to the gingiva. Score 1: There is a plaque in the form of a thin film on the gingival margin. Score 2: There is a visible plaque in the gingival pocket and gingival margin. Score 3: There is a dense plaque in the gingival pocket and on the gingival margin. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Salivary Malondialdehyde biomarker | Baseline:saliva collection for malondialdehyde . up to two hours fasting for all subjects. | Measure salivary malondialdehyde biomarker for all 90 subjects (computed tomography,x\_ray and laboratory group) to see if the ionizing radiation effect on salivary Redox status in relation to oral health condition for workers in radiation field |
| Salivary 8_ hydroxy_deoxyguanosine biomarker | Baseline:saliva collection for 8_hydroxy_deoxyguanosine .up to two hours fasting for all subjects. | Measure salivary 8\_hydroxy\_deoxyguanosine biomarker for all 90 subjects (computed tomography,x\_ray and laboratory group) to see if the ionizing radiation effect on salivary Redox status in relation to oral health condition for workers in radiation field |
| Salivary catalase biomarker | Baseline:saliva collection for catalase . up to two hours fasting for all subjects. | Measure salivary catalase biomarker for all 90 subjects (computed tomography,x\_ray and laboratory group) to see if the ionizing radiation effect on salivary Redox status in relation to oral health condition for workers in radiation field |
Contacts
Primary ContactJihan Munther Hussien, B.D.S
Outcome results
None listed