Pseudomonas Aeruginosa Infection, Rapid Detection
Conditions
Brief summary
The aim of this study is to observe specimens of bronchoalveolar lavage fluid from patients using a reflection enhanced dark-field scattering microscopy. By observing parameters such as the size, morphology, scattering intensity, and movement speed of pathogens, combined with the clinical characteristics of patients, a rapid diagnosis of Pseudomonas aeruginosa infection can be made. At the same time, the bronchoalveolar lavage fluid samples of patients infected with Pseudomonas aeruginosa before and after treatment were compared, and the quantity and vitality of Pseudomonas aeruginosa were observed to judge the efficacy of antibiotics.
Interventions
PROCEDUREbronchoscopy
We will perform bronchoscopy and collect bronchoalveolar lavage fluid (BALF)
Sponsors
The First Affiliated Hospital with Nanjing Medical University
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 90 Years
Healthy volunteers
No
Inclusion criteria
* Aged 18 years and above * After admission, Pseudomonas aeruginosa was cultured in bronchoalveolar lavage fluid or lower respiratory tract aspirates
Exclusion criteria
* Age under 18 years old or over 90 years old * Pregnancy, lactation period -
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| The size of pathogens | From enrollment to the end of treatment at 2 weeks | Measure the size of pathogens |
| scattering intensity of pathogen | From enrollment to the end of treatment at 2 weeks | Determine the shape of pathogens through scattering intensity |
| movement speed of pathogens | From enrollment to the end of treatment at 2 weeks | Measure the movement speed of pathogens, such as flagellates like Pseudomonas aeruginosa, which have a fast movement speed |
Countries
China
Contacts
Primary ContactWenkui Sun, Dr
Outcome results
None listed