Metabolic Disease, Obesity, Obesity Prevention
Conditions
Keywords
lifestyle intervention, exerkines, metabolic flexibility
Brief summary
The goal of this clinical trial is to detect the impact of the 3-month complex lifestyle intervention, consisting of dietary counselling, supervised exercise training and cognitive-behavioural intervention in middle-aged sedentary individuals with obesity, specific questions are: * What is the adaptive response to exercise training on the whole-body and skeletal muscle energy metabolism, insulin sensitivity, metabolic flexibility, muscle functional parameters and cardiopulmonary fitness? * Would the intensity-related changes of the exercise-induced substrate preference (lipids vs carbohydrates) be a good predictive marker of metabolic flexibility i.e. the capacity to reduce the whole-body metabolic burden in patients with obesity? * Is the signal driving the adaptive response to regular exercise contained in serum derived Extracellular vesicles? Researchers will examine effects of exercise training intervention on whole body energy metabolism, by integrating clinical, biochemical and molecular data. Participants will: * Undergo 3 months exercise - intervention program. * Be submitted to examination of their obesity, energy metabolism and cardiopulmonary fitness related phenotypes using bioelectric impedance, indirect calorimetry, cycle spiroergometry. * Blood samples will be taken both, at the baseline PRE- and POST- intervention.
Interventions
BEHAVIORALsupervised lifestyle modification
Supervised lifestyle intervention will consist of regular exercise (3x a week), nutritional counselling (weekly), cognitive bahavioural therapy (monthly).
Sponsors
Medical University Vienna
Slovak Academy of Sciences
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
20 Years to 50 Years
Healthy volunteers
No
Inclusion criteria
BMI 30-45 kg.m-2 -
Exclusion criteria
smoking, cancer, \-
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Metabolic flexibility in response to submaximal exercise and nutritional challenge | 24 months | Metabolic flexibility (metabolic substrate switching) will be measured using indirect calorimetry during submaximal exercise test (on stationary bike) and in response to nutritional challenge (fasting / glucose uptake during oGTT) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| cardiopulmonary fitness (VO2max) | 24 months | Cardiopulmonary fitness will be measured by bicycle spiro-ergometry |
| Index of insulin resistance (oGTT) | 24 months | Index of insulin resistance will be calculated from the results of the frequently sampled oral glucose tolerance test oGTT. |
| abdominal adiposity and abdominal adipose tissue distribution (subcutaneous / visceral) | 24 months | abdominal adiposity will be assessed by quadrupedal biopimpedance, and adipose tissue distribution by magnetic resonance imagining |
Countries
Slovakia
Outcome results
None listed