Evaluation of the Salivary-free Amino Acids Levels (Glycine , Proline , Lysine , and Alanine ) With Caries Activity

Evaluation of the Salivary-free Amino Acids Levels With Caries Activity

Status

Not yet recruiting

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT06953557

Enrollment

Registered

2025-05-01

Start date

2025-04-30

Completion date

2025-06-30

Last updated

2025-05-01

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Dental Caries

Keywords

Salivary -free amino acid measurment

Brief summary

This study will be aimed to investigated the correlation between levels of salivary amino acids (glycine , proline , lysine , alanine ) in the saliva of healthy children with and without active carious lesions and to estimate the predictive capacity of caries susceptibility

Detailed description

80 healthy children (8-10 years old) will be recruited from primary schools of Baghdad city/ Iraq, will be classified according to their level of caries activity (high caries activity, n = 40; negative caries activity, n = 40). Total stimulated and unstimulated saliva will be collected to estimate the caries activity by using the Snyder test, and Amino acid analysis of the saliva will be performed by reversed phase - high performance liquid chromatography (RP-HPLC), respectively.

Interventions

DIAGNOSTIC_TESTTo compare the effect of salivary glycine , proline , lysine and alanine concentration between participants with high caries activity and participants with negative caries activity

The investigators will measure Salivary-free amino acids by using reverse phase high performance liquid chromatography

Study design

Observational model

CASE_CONTROL

Time perspective

CROSS_SECTIONAL

Eligibility

Sex/Gender

ALL

Age

8 Years to 10 Years

Healthy volunteers

Yes

Inclusion criteria

* Children aged (8,9,10) years with different caries * Absence of signs of gingivitis or periodontal disease.

Exclusion criteria

* Children out the range of age * Children with any systemic discases. * Children with appliances in the mouth. * Children with appliances in the mouth. * Children with antibiotic treatment or any other drug over the last two weeks. B

Design outcomes

Primary

Measure	Time frame	Description
Salivary-free amino acid analysis	Baseline :unstimulated saliva collection for salivary glycine , proline , lysine and alanine measurement	Unstimulated saliva collection for salivary free amino acids analysis

Contacts

Primary ContactAbeer Ibrahim Mohammed, B.D.S

abeeribrahim93@uomustansiriyah.edu.iq+9647709497799

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026