Healthy, Respiratory Quotient Measurement
Conditions
Brief summary
This trial evaluates the effect of molecular hydrogen inhalation on metabolic response, heart rate, and rating of perceived exertion in healthy females during 30 minutes of exercise on a bicycle ergometer.
Detailed description
The objective of this randomized, double-blind, placebo-controlled crossover trial is to assess the effect of molecular hydrogen inhalation on metabolic response during 20 min of rest and during exercise on a bicycle ergometer with a workload of 0.5 W/kg for 10 min, 0.75 W/kg for 10 min, and 1 W/kg for 10 min. The trial recruits healthy females aged 20-35 years and the planned sample size is 20. The trial consists of two experimental sessions, one using molecular hydrogen inhalation and the other using placebo inhalation. The washout period between sessions is one week. Randomization of the order of the sessions is performed using a computer random generator. Molecular hydrogen is produced using a hydrogen generator via electrolysis of water and administered through a mouthpiece. Ambient air is used for placebo inhalation. Molecular hydrogen is colorless, odorless, and tasteless, making it indistinguishable from placebo by the human senses. During the session, the following variables are measured: ventilation, oxygen uptake, respiratory exchange ratio, heart rate, rating of perceived exertion, and oxygen saturation. Energy expenditure, fat oxidation rate and carbohydrates oxidation rate are calculated from oxygen uptake and respiratory exchange ratio. Statistical analysis is performed at the 0.05 significance level and data are evaluated using analysis of variance for repeated measures with Fisher's post hoc tests.
Interventions
OTHERMolecular hydrogen
Hydrogen gas, purity \>99.9%, flow rate 600 ml/min (two generators 300 ml/min each).
OTHERPlacebo
Ambient air, flow rate 600 ml/min (two pumps 300 ml/min each).
Sponsors
Palacky University
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* Free of major diseases. * Signed informed consent.
Exclusion criteria
* Acute conditions contraindicating laboratory testing of exercise. * Cardiorespiratory disease. * Pharmacotherapy affecting metabolism. * Musculoskeletal problems.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Respiratory exchange ratio | Change after 1 week of crossover. | Respiratory exchange ratio is measured with Ergostik analyser (Geratherm Respiratory, Bad Kissinger, Germany) breath by breath during the session and averaged over 5 min windows for subsequent analysis. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Ventilation | Change after 1 week of crossover. | Ventilation is measured with Ergostik analyser (Geratherm Respiratory, Bad Kissinger, Germany) breath by breath during the session and averaged over 5 min windows for subsequent analysis. |
| Oxygen uptake | Change after 1 week of crossover. | Oxygen uptake is measured with Ergostik analyser (Geratherm Respiratory, Bad Kissinger, Germany) breath by breath during the session and averaged over 5 min windows for subsequent analysis. |
| Heart rate | Change after 1 week of crossover. | Heart rate is recorded by Polar H10 chest strap (Polar, Kempele, Finland) during the session and averaged over 5 min windows for subsequent analysis. |
| Rating of perceived exertion | Change after 1 week of crossover. | Participants are asked to subjectively rate their perceived exertion at specified time points (at the end of rest, after 10, 20, and 30 min of exercise). The Borg's scale 6 (no exertion) and 20 (maximal exertion) is used. |
Countries
Czechia
Outcome results
None listed